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SyliteD1.1 anti-Gephyrin

Immunostaining of PFA fixed mouse hippocampus neurons with FluoTag®-X2 anti-VGlut1 Atto 488 (Cat. No. N1602-At488-L, dilution 1:500, represented in yellow) and SyliteD1.1 anti-Gephyrin-SC5 (Cat No. P4001-SC5) dilution 1:250, represented in cyan.

3D MINFLUX with SyliteD1.1 SC5 (Cat No. P4001-SC5). Courtesy of Elisa D´Este, MPI for Medical Research, Heidelberg, Germany.
SyliteD1.1 anti-Gephyrin

SyliteD1.1 anti-Gephyrin

Cat No: P4001 Category:

325,00 €

SyliteD1.1 anti-Gephyrin is a short dimeric peptidic probe that specifically binds gephyrin in inhibitory synapses. We provide SyliteD1.1 anti-Gephyrin fluorescently labeled with sulfo-cyanine 5 (SC5). In super-resolution microscopy, SyliteD1.1 enables accurate localization of inhibitory synapses and supports nanoscale measurements. The SyliteD1.1 peptide contains extra lysines to allow post-staining fixation in place.

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SyliteD1.1 anti-Gephyrin is a short dimeric peptidic probe that specifically binds gephyrin in inhibitory synapses. We provide SyliteD1.1 anti-Gephyrin fluorescently labeled with sulfo-cyanine 5 (SC5). SyliteD1.1 anti-Gephyrin was developed by Hans Maric and Vladimir Khayenko from the University of Würzburg with the goal to produce a probe with an outstanding signal-to-background ratio that outperforms antibodies in tissue staining with rapid and efficient penetration, mitigation of staining artifacts, and simplified handling. In super-resolution microscopy, SyliteD1.1 precisely localizes the inhibitory synapse and enables nanoscale measurements. The SyliteD1.1 peptide contains extra lysines to allow post-staining fixation in place.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) with SyliteD1.1
Variations:
Conjugation Amount Cat No. RRID
200 μl Sulfo-Cyanine 5 P4001-SC5 -
Related Products: View related products
Clone: N/A
Host: -
Produced in: Synthetic peptide
Application: IF, IHC
Dilution: 1:250
Capacity: -
Antigen: -
Targets: Gephyrin
Specificity: SyliteD1.1 anti-Gephyrin specifically recognizes mouse and rat gephyrin. Other species not tested.
Formulation:

Lyophilized from 200 µL at 5 µM SyliteD1.1. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: SyliteD1.1 SC5 - 3424 Da
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored for up to 4 weeks at -20°C when reconstituted in 50% glycerol/PBS or at 4°C when reconstituted in DMSO. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

When preparing samples for mounting with media such as Mowiol, we recommend a post-fixation step after incubation with SyliteD1.1 SC5. For more details, click the Protocols button above or visit our Resources page.
References:
  1. Khayenko V, Schulte C, Reis SL, et al. A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity. Angew Chem Int Ed Engl. 2022;61(30):e202202078. doi:10.1002/anie.202202078
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infect
Legal terms: By purchasing this product you agree to our general terms and conditions.