Immunostaining of PFA fixed mouse hippocampus neurons with FluoTag®-X2 anti-VGlut1 Atto 488 (Cat. No. N1602-At488-L, dilution 1:500, represented in yellow) and SyliteD1.1 anti-Gephyrin-SC5 (Cat No. P4001-SC5) dilution 1:250, represented in cyan.
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3D MINFLUX with SyliteD1.1 SC5. Courtesy of Elisa D´Este, MPI for Medical Research, Heidelberg, Germany.
SyliteD1.1 anti-Gephyrin
325,00 €
SyliteD1.1 anti-Gephyrin is a short dimeric peptide that specifically binds gephyrin in inhibitory synapses. We supply SyliteD1.1 anti-Gephyrin fluorescently labelled with sulfo-cyanine 5 (SC5). SyliteD1.1 anti-Gephyrin was developed by Hans Maric and Vladimir Khayenko from the University of Würzburg with the goal to produce a probe with an outstanding signal-to-background ratio that outperforms antibodies in tissue staining with rapid and efficient penetration, mitigation of staining artifacts, and simplified handling. In super-resolution microscopy, SyliteD1.1 precisely localizes the inhibitory synapse and enables nanoscale measurements. The SyliteD1.1 peptide contains extra lysines to allow post-staining fixation in place.
SyliteD1.1 anti-Gephyrin is a short dimeric peptide that specifically binds gephyrin in inhibitory synapses. We supply SyliteD1.1 anti-Gephyrin fluorescently labelled with sulfo-cyanine 5 (SC5). SyliteD1.1 anti-Gephyrin was developed by Hans Maric and Vladimir Khayenko from the University of Würzburg with the goal to produce a probe with an outstanding signal-to-background ratio that outperforms antibodies in tissue staining with rapid and efficient penetration, mitigation of staining artifacts, and simplified handling. In super-resolution microscopy, SyliteD1.1 precisely localizes the inhibitory synapse and enables nanoscale measurements. The SyliteD1.1 peptide contains extra lysines to allow post-staining fixation in place.
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| Related Products: | - | ||||||||
| Clone: | N/A | ||||||||
| Host: | - | ||||||||
| Produced in: | Synthetic peptide | ||||||||
| Application: | IF, IHC | ||||||||
| Dilution: | 1:250 | ||||||||
| Capacity: | - | ||||||||
| Antigen: | - | ||||||||
| Targets: | Gephyrin | ||||||||
| Specificity: | SyliteD1.1 anti-gephyrin specifically recognizes mouse and rat gephyrin. Other species not tested. | ||||||||
| Formulation: | Lyophlized from 200 µL at 5 µM SyliteD1.1. | ||||||||
| kDa: | SyliteD1.1 SC5 - 3424 Da | ||||||||
| Ext Coef: | - | ||||||||
| Shipping: | Ambient temperature | ||||||||
| Storing: | Vials containing lyophilized peptide can be stored at 4 °C for 6 months. We recommend reconstituting the peptide with DMSO if applicable. The peptide will also be solubilzed in PBS. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted peptide. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted peptide at 4 °C. | ||||||||
| Protocols: |
When samples are intended to be mounted with for example Mowiol, we recommend a post-fixation step after the incubation with the SyliteD1.1 SC5. If you follow the IF protocol in our resource Section the additional post-fixation step uses 4% PFA buffered in PBS. |
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| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infect | ||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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