275,00 € – 625,00 €
FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of murine Synaptotagmin 1 with high affinity and specificity.
Synaptotagmin 1 (Syt1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion. Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.
Our nanobody recognizes strongly and specifically the cytosolic portion of SYT1.
Variations: |
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Related Products: | - | ||||||||||||||||
Clone: | 7D5 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: | IF | ||||||||||||||||
Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | - | ||||||||||||||||
Targets: | Synaptotagmin 1 (cytosolic) | ||||||||||||||||
Specificity: | Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested). | ||||||||||||||||
Formulation: | DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water (unconjugated sdAbs) or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. | ||||||||||||||||
kDa: | 15,5 | ||||||||||||||||
Ext Coef: | 24200 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | ||||||||||||||||
Protocols: |
This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly. Look at detailed protocols in our Resource Section. |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |