Immunostaining of PFA fixed rat hippocampus neurons with FluoTag-X2 anti-Synaptotagmin-1 Atto 488 (Cat. No. N2302-At488-L, dilution 1:500; green) and anti-Gephyrin Cy3 (Synaptic Systems Cat. No. 147 011C3; red). Nuclei were visualized with DAPI (blue).

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Alexa Fluor 647 (Cat. No. N2302-AF647, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF647. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-At488. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in magenta, B in green and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to AZDye 568 (Cat. No. N2302-AF568, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF568. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in red, B in magenta and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Atto 488 (Cat. No. N2302-At488, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-At488. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in green, B in magenta and C in blue.