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Immunostaining of PFA fixed rat hippocampus neurons with FluoTag-X2 anti-Synaptotagmin-1 Atto 488 (Cat. No. N2302-At488-L, dilution 1:500; green) and anti-Gephyrin Cy3 (Synaptic Systems Cat. No. 147 011C3; red). Nuclei were visualized with DAPI (blue).

 FluoTag-X2 anti-SYT1 AF647

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Alexa Fluor 647 (Cat. No. N2302-AF647, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF647. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-At488. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in magenta, B in green and C in blue.

 FluoTag-X2 anti-SYT1 AF568

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to AZDye 568 (Cat. No. N2302-AF568, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF568. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in red, B in magenta and C in blue.

 FluoTag-X2 anti-SYT1 At488

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Atto 488 (Cat. No. N2302-At488, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-At488. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in green, B in magenta and C in blue.
FluoTag-X2 anti-SYT1 AF647
FluoTag-X2 anti-SYT1 AF568
FluoTag-X2 anti-SYT1 At488

sdAb anti-Synaptotagmin 1 (7D5)

Cat No: N2305 Category:

275,00 € – 625,00 €

A single-domain antibody (sdAb) that specifically and strongly binds to the cytoplasmic domain of murine and rat Synaptotagmin 1 (SYT1).

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Synaptotagmin 1 (SYT1) is a member of a protein family consisting of 17 members. Synaptotagmins are single-pass transmembrane proteins containing two calcium-binding domains in their intracellular portion. Synaptotagmin 1 is enriched at the presynaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase in calcium concentration.

Our nanobody strongly and specifically binds to the cytosolic portion of mouse and rat SYT1.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Biotin 250 μg N2305-Biotin -
DBCO 250 μg N2305-DBCO -
unconjugated C-terminal Cys 250 μg N2305-250ug -
Related Products: -
Clone: 7D5
Host: Alpaca
Produced in: E.coli
Application:

IF

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested).
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4.
Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0.
Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

kDa: 15,5
Ext Coef: 24200
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References:
  1. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; primary neurons and synaptic vesicles from rat)
  2. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF, IHC; rat, mouse)
  3. Hoffmann C, Rentsch J, Tsunoyama TA, et al. Synapsin condensation controls synaptic vesicle sequestering and dynamics. Nat Commun. 2023;14(1):6730. Published 2023 Oct 23. doi:10.1038/s41467-023-42372-6 (ICC/IF; mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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