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Immuno-histochemical sections of FFPE Covid-19 infected-Vero cells were kindly provided by Prof- Dr. med. Andreas C. Hocke (Charité -Universitätsmedizin Berlin). Staining with biotinylated sdAb anti-SARS-CoV-2 Nucleocapsid (N3505-Biotin) and 1:100 visulized with ABC Kit HRP (Vector PK-4000). Nuclei have been counterstained with haematoxylin (blue).

sdAb anti-SARS-CoV-2 Nucleocapsid

Cat No: N3605 Category:

275,00 € – 625,00 €

A single-domain antibody (sdAb) that strongly binds to the nucleocapsid (N) protein of SARS-CoV-2.

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The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.

Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g. paraffin-embedded tissue.

MSDS
Protocols
Reconstitution and Storage
Maleimide Labeling
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N3605-250ug AB_3076099
Biotin 250 μg N3605-Biotin AB_3076100
DBCO 250 μg N3605-DBCO AB_3076101
Related Products:

FluoTag®-Q anti-SARS-CoV-2 Nucleocapsid (Cat. No. N3601)

sdAb anti-SARS-CoV-2 Spike protein S1 (Cat. No. N3505)

FluoTag®-Q anti-SARS-CoV-2 Spike protein S1 (Cat. No. N3501)

Recombinant anti-SARS-CoV-2 Spike protein S1 antibody (rHcAb) (Cat. No. N3583)

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Clone: 2A7
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: SARS-CoV-2 Nucleocapsid
Specificity: Recognizes the Nucleocapsid protein of SARS-CoV-2 and SARS-CoV-1.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 16.8
Ext Coef: 30098
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.