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sdAb anti-Mouse IgG1

Cat No: N2005 Category:

275,00 € – 625,00 €

sdAb anti-Mouse IgG1 (Smart Secondaries®) is a single-domain antibody (sdAb) that binds specifically and strongly to mouse IgG1. This makes it an ideal secondary tool for demanding immunolabeling and imaging applications.

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Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either lambda or kappa light chain.

sdAb anti-Mouse IgG1 binds specifically and strongly to mouse IgG1 independently of the IgGs light chains.

To learn more about the Smart Secondaries®, please visit our Technology section here.

We provide this product conjugated to various labels and offer custom conjugation services with most commercially available labels, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Maleimide labeling
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N2005-250ug AB_3076025
Biotin 250 μg N2005-Biotin AB_3076026
DBCO 250 μg N2005-DBCO AB_3076027
HRP 200 μl N2005-HRP AB_3076028
Related Products: -
Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application: IF, IHC, WB
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. The HRP-conjugated sdAbs are lyophilized from HRP protector buffer and should be reconstituted with 200 µL pure deionized water for storage at 4°C or with 200 µL high purity, sterile 50% glycerol for storage at -80°C. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.
kDa: 15.9
Ext Coef: 24138
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks (Un-, DBCO-, Biotin-conjugated sdAbs) or at 4°C for up to 6 months (HRP-conjugated sdAbs). For more details, click the “Protocols” button above and check “Reconstitution and Storage”.
Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References:
  1. Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
  2. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT; rat)
  3. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; rat primary neurons)
  4. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (WB; mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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