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sdAb anti-Mouse IgG1

Cat No: N2005 Category:

275,00 € – 625,00 €

sdAb anti-Mouse is a species-specific sdAb directed against mouse IgG1.

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Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either lambda or kappa light chain.

Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N2005-250ug AB_3076025
Biotin 250 μg N2005-Biotin AB_3076026
DBCO 250 μg N2005-DBCO AB_3076027
HRP 200 μl N2005-HRP AB_3076028
Related Products: -
Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application: IF, IHC, WB
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. HRP-conjugated sdAbs are lyophilized from HRP-Protector. Reconstitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. Reconstitute HRP-conjugated sdAbs with 200 µL pure water.
kDa: 15.9
Ext Coef: 24138
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols: -
References:
  1. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT; rat)
  2. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; rat primary neurons)
  3. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (WB; mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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