275,00 € – 625,00 €
sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several enhanced versions have been discovered expanding the repertoire of photoswitchable fluorescent probes. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”
Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Check out our Specificity Chart for more details.
Variations: |
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Related Products: | - | ||||||||||||||||
Clone: | 1E8 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: |
IF (ICC) IHC Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | - | ||||||||||||||||
Targets: | mEOS4b | ||||||||||||||||
Specificity: | Recognizes mEOS and mEOS derivatives. | ||||||||||||||||
Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the “Protocols” button above and check “Reconstitution and Storage”. |
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kDa: | 16.4 | ||||||||||||||||
Ext Coef: | 24598 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storing: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the “Protocols” button above and check “Reconstitution and Storage”. |
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Protocols: |
Relevant protocols can be found under the “Protocols” button above. For additional information, visit our our Resources page and Specificity Chart. |
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References: | - | ||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |