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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEos reporter protein with FluoTag®-X2  anti-mEos Alexa Fluor 647 (Cat. No. N3102-AF647-L, dilution 1:500). Nuclei were visualized by DAPI staining (blue).

sdAb anti-mEos

Cat No: N3105 Category:

275,00 € – 625,00 €

sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.

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In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.

Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Have a look at our specificity chart in the resource section.

 

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N3105-250ug AB_3076066
Biotin 250 μg N3105-Biotin AB_3076067
DBCO 250 μg N3105-DBCO AB_3076068
Related Products: -
Clone: 1E8
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mEOS4b
Specificity: Recognizes mEOS and mEOS derivatives.
Formulation: DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.
kDa: 16.4
Ext Coef: 24598
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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