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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEos reporter protein with FluoTag®-X2  anti-mEos Alexa Fluor 647 (Cat. No. N3102-AF647-L, dilution 1:500). Nuclei were visualized by DAPI staining (blue).

sdAb anti-mEos

Cat No: N3105 Category:

Price range: 290,00 € through 655,00 €

sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii. Available conjugated to Biotin, DBCO, C-terminal Cysteine, or customized upon request. For fluorescent conjugates, see our FluoTag® product line.

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In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several enhanced versions have been discovered expanding the repertoire of photoswitchable fluorescent probes. An open-source database with the most currently available variants can be found at the Fluorescent Protein Database FPbase.

Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Check out our FP Specificity Chart for more details.

 

Documents
sdAb unconjugated
sdAb-Biotin/DBCO
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N3105-250ug AB_3076066
Biotin 250 μg N3105-Biotin AB_3076067
DBCO 250 μg N3105-DBCO AB_3076068
Related Products:

FluoTag®-X2 anti-mEos (Cat. No. N3102)

View related products
Clone: 1E8
Host: Alpaca
Produced in: E.coli
Application:

IF (ICC)

IHC

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mEOS4b
Specificity: Recognizes mEOS and mEOS derivatives.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 16.4
Ext Coef: 24598
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

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