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Live Ramos B cells stained with anti-Human IgM single-domain antibody conjugated to Atto 647N.

sdAb anti-Human IgM

Cat No: N1101 Category:

550,00 €

sdAb anti-Human IgM is an in-house developed single-domain antibody binding human IgM on cell surfaces (e.g., as B cell receptor) as well as in serum with high affinity and specificity.

 

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The anti-Human IgM single-domain antibody is an in-house developed camelid sdAb that specifically recognizes human IgM. It has no cross-reactivity with human IgG molecules. This product can be fully customized to meet your needs, for example, sdAb anti-Human IgM is also available as MBP (maltose binding protein) fusion protein to increase its size, ideal for immobilizing it in lateral flow assays.

 

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 1 mg N1101-1mg AB_3075953
MBP fusion 1 mg N1105-1mg AB_3075954
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Clone: 2F2
Host: Alpaca
Produced in: E.coli
Application: ELISA, FACS, IF, IP
Dilution: N/A
Capacity: N/A
Antigen: IgM
Targets: IgM
Specificity: Specific for Human IgM. No cross-reactivity to Human IgG, IgE or IgA. No significant cross-reactivity to IgM from Mouse, Guinea pig or Rabbit. Other species not tested.
Formulation:

Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. sdAbs-MBP fusion are lyophilized from PBS pH 7.4. Reconstitute with 500 µL pure deionized water (Unconjugated sdAbs for immediate labelling with maleimide compounds) or with 500 µL sterile 50% glycerol for storage at -80°C (sdAbs-MBP fusion). For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 15.7 (N1101) and 55.7 (N1105)
Ext Coef: 21618 (N1101) and 87905 (N1105)
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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