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Human PBMCs isolated from buffy coat using a Ficoll gradient
and co-staining with anti-CD4-APC, anti-CD62L-PE (DREG-56)
and our FluoTag-X2 anti-CD62L-FITC.

sdAb anti-CD62L

Cat No: N1405 Category:

275,00 € – 625,00 €

sdAb anti-CD62L is an in-house developed single-domain antibody (sdAb) binding the human lymphocyte marker CD62L (L-selectin) with high affinity and specificity.

 

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CD62L, or L-selectin is a surface marker protein present on naïve T cells. Its regulation plays a pivotal role in controlling the traffic of T-lymphocytes to and from peripheral lymph nodes. Compared to other T-cell surface marker proteins, such as CCR7, which can be found in either in central memory cells (Tcm), or in effector memory cells (Tem), CD62L is uniformly expressed in Tcm cells (CD62L+), allowing for a clear differentiation between T-cell populations.

 

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N1405-250ug AB_3075976
Biotin 250 μg N1405-Biotin AB_3075977
DBCO 250 μg N1405-DBCO AB_3075978
Related Products: -
Clone: P3C11
Host: Alpaca
Produced in: E.coli
Application: FACS, IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: CD62L
Specificity: Recognizes the extracellular domain of human CD62L (L-selectin).
Formulation: DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the "Protocols" button above and check "Reconstitution and Storage".
kDa: 16.3
Ext Coef: 31128
Shipping: Ambient temperature
Storing: Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the "Protocols" button above and check "Reconstitution and Storage".
Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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