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Indirect immunostaining of PFA fixed COS cells expressing POM121-GFP with recombinant anti-GFP antibody (Cat. No. N0383; 1:500) and decorated with FluoTag®-X4 anti-Rabbit AZDye 568 (Cat. No. N2404-AF568-S; dilution 1:500), the merge of both channels is represented in orange. Nuclei were visualized by DAPI staining (blue).

Recombinant anti-GFP antibody (rHcAb)

Cat No: N/A Category:

420,00 €

The recombinant heavy-chain anti-GFP antibody is a genetic fusion of our established camelid anti-GFP single-domain antibody (sdAb) to a Rabbit IgG Fc domain.

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The recombinant heavy-chain anti-GFP antibody is a genetic fusion of our established camelid anti-GFP single-domain antibody (sdAb) to a Rabbit IgG Fc domain. This antibody preserves the specificity and high-affinity binding of the anti-GFP sdAb and adds the features of a conventional rabbit antibody. It can be used in many applications based on immunodetection (IF, IHC, IP). However, this recombinant anti-GFP antibody will only recognize folded GFP and therefore will not work in Western blotting.

Our recombinant anti-GFP antibody binds strongly to most fluorescent proteins derived from Aequorea victoria. Explore our FP Specificity Chart for compatibility information.

More information on recombinant heavy-chain antibodies (rHcAb) can be found here.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Rabbit IgG Fc-fusion 100 µg N0383 AB_3075913
Related Products:

FluoTag®-Q anti-GFP (Cat. No. N0301)

FluoTag®-X4 anti-GFP (Cat. No. N0304)

sdAb anti-GFP (Cat. No. N0305)

GFP Selector (Cat. No. N0310; N0315)

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Clone: 1H1
Host: Alpaca
Produced in: HEK
Application: IF, IHC
Dilution: 1:500 (IF, IHC)
Capacity: N/A
Antigen: -
Targets: GFP
Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
kDa: -
Ext Coef: -
Formulation:

Lyophilized from PBS pH 7.4. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.
Product LULL: Limited Use Label License (LULL) – Internal Research Use Only applies.