Indirect immunostaining of PFA-fixed, paraffin embedded mouse brain olfactory bulb section with recombinant anti-SARS-CoV-2 Spike antibody (N3583) (dilution 10 µg/mL). Secondary reagent biotinylated anti-rabbit (Jackson 712-065-153) and visualized by ABC Kit HRP Standard (Vector PK-4000).
Nuclei have been visualized by haematoxylin staining (blue).
SARS-Cov-2 Spike S1
Coronaviruses form trimers with the spike glycoprotein (S) to bind its receptor, mediate membrane fusion, and result in the virus entry. Each spike glycoprotein monomer S is about 180 kDa and contains two subunits, the S1 and S2, mediating attachment and membrane fusion, respectively. The S1 subunit of S protein folds in 2 main domains, the N-terminus domain (NTD) and the receptor-binding domain (RBD), which is responsible for binding a surface protein on mammalian cells.
Our sdAb binds to the S1 protein and is able to neutralize the virus entry by blocking the interaction between RDB and the cell surface-exposed receptor.
The recombinant sdAb anti-SARS-CoV-2 Spike protein S1 Rabbit Fc-fusion is a genetic fusion of the single-domain antibody anti-SARS-CoV-2 Spike protein S1 to a Rabbit Fc domain. This bivalent tool binds the SARS-CoV-2 RBD domain of the S1 protein with high affinity and is ideally suited for ELISA and IHC staining. The fused Rabbit Fc enables researchers to employ conventional secondary detection tools commonly used in automated high-throughput-screening devices. The recombinant heavy-chain antibody preserves all the biophysical features of the high-affinity binding of the anti-S1 SARS-CoV-2 single-domain antibody and is also capable of neutralizing infections by competing with ACE-2 receptor binding as previously demonstrated by the team of Alejandro Rojas-Fernandez at the Institute of Medicine from the Universidad Austral de Chile.
|Dilution:||1:500 (IF), 10 µg/mL (IHC), 1:1000 ELISA|
|Specificity:||Recognizes the RBD of the SARS-CoV-2 S1 protein.|
|Formulation:||Lyophilized from PBS pH 7.4, containing 100 µg rec. hcAbs and 2% BSA (US-Origin). Reconstitute with 100 µL 50% glycerol. We recommend including 0.1 % sodium azide as a preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.|
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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