FluoTag®-X4 anti-GFP abberior STAR_STED-optimized.png

Confocal vs. STED images of PFA-fixed U2OS-NUP96-GFP stable cell line stained with FluoTag®-X4 anti-GFP conjugated to abberior STAR RED (left panel, Cat. No. N0304-AbRED-L) or abberior STAR ORANGE (right panel, Cat. No. N0304-AbORANGE-L). FluoTag®-X4 anti-GFP specifically binds to GFP fused to NUP96, enabling the visualization of NUP96 nucleoporins within the Nuclear Pore Complex (NPC). The STED images reveal fine structural details with superior resolution compared to confocal microscopy. Images acquired by abberior.

dSTORM image of immunostaining of Cos7 cells expressing TOM70-EGFP fusion protein. TOM70-EGFP reporter was labeled with FluoTag®-X4 anti-GFP coupled to Janelia Fluor® 635b (Cat. No. N0304-JF635B-L). Image courtesy of R. Tsukanov and S. Basak, Institute of Physics – Biophysics, Georg August University, Göttingen, Germany.

Indirect immunostaining of PFA fixed Hela cells expressing Nup98-GFP with FluoTag®-X4 Atto 565 anti-GFP sdAb (Cat. No. N0304, dilution 1:500, the GFP signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow).

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to Alexa Fluor 647 (Cat. No. N0304-AF647, dilution 1:500). A Greyscale image of the staining performed with N0304-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to AZDye568 (Cat. No. N0304-AF568, dilution 1:500). A Greyscale image of the staining displayed with N0304-AF568. B False color representation of the image shown in A is presented in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to Atto 488 (Cat. No. N0304-At488, dilution 1:500). A Greyscale image of the staining performed with N0304-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-X4 anti-GFP

Name: FluoTag®-X4 anti-GFP
SKU: N0304
Price: 400.00 EUR
Availability: InStock

Cat No: N0304 Category: Primary sdAbs

400,00 €

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. It enables direct and highly specific fluorescent detection of GFP-tagged targets without the need for secondary antibodies.

Clear

General information: A breakthrough in biology and bioluminescence began in the 1960s when Osamu Shimomura and colleagues discovered a glowing protein in the jellyfish Aequorea victoria. This paved the way for fluorescence-based research. However, it wasn’t until the early 1990s that the green fluorescent protein (GFP) sequence was cloned and used as a fluorescent marker in foreign organisms. Today, more than 800 fluorescent protein variants are cataloged in open-source databases, with the most extensive collection available on FPbase.

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs), each binding to distinct epitopes on GFP. Every sdAb carries two fluorophores, decorating GFP-tagged targets with a total of four fluorophores (FluoTag®-X4). This design enhances brightness and detection sensitivity. For more details on our FluoTag®-Q, -X2, and -X4 series, visit our Technology Section.

FluoTag®-X4 anti-GFP binds strongly to most fluorescent proteins derived from Aequorea victoria. Explore our FP Specificity Chart for compatibility information.

NEW: Learn more about our latest variants conjugated to abberior STAR dyes, optimized for STED and confocal microscopy.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
abberior STAR 635P	200 μl	N0304_Ab635P-L	AB_3075902
abberior STAR 460L	200 μl	N0304-Ab460L-L	AB_3678866
abberior STAR GREEN	200 μl	N0304-AbGREEN-L	AB_3678867
abberior STAR ORANGE	200 μl	N0304-AbORANGE-L	AB_3678868
abberior STAR RED	200 μl	N0304-AbRED-L	AB_3678869
Janelia Fluor 635b	200 μl	N0304-JF635B-L	AB_3668671
Atto488	200 μl	N0304-At488-L	AB_2744629
AZDye568	200 μl	N0304-AF568-L	AB_3075903
Atto643	200 μl	N0304-At643-L	AB_3075906
Alexa647	200 μl	N0304-AF647-L	AB_2905517

FluoTag®-X4 anti-GFP

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