IHC on formalin-fixed paraffin-embedded (FFPE) tissue. Chromogenic IHC staining of a rabbit anti-PSD95 r-hcAb (Cat. No. N3783, dilution 1 μg/ml) in mouse retina using 3,3′-Diaminobenzidin (DAB; brown staining). Hematoxylin provides blue nuclear staining. Intense DAB staining is present in the outer plexiform layer (OPL). Moderate punctate staining is observed in the inner plexiform layer (IPL). The inner and outer nuclear layers (INL and ONL) are not stained. Figure modified from Kilisch et al., 2023 (see References below).

IHC on formalin-fixed paraffin-embedded (FFPE) tissue. Chromogenic IHC staining of a rabbit anti-PSD95 r-hcAb (Cat. No. N3783, dilution 1 μg/ml) in mouse cerebellum using 3,3′-Diaminobenzidin (DAB; brown staining). High-magnification view of mouse cerebellum sections. Hematoxylin provides blue nuclear staining.  Intense PSD95 staining is detected within the basket cell terminal pinceaux (arrows). Purkinje cells (P) and neurons in the granule layer are not stained. Figure modified from Kilisch et al., 2023 (see References below).

Detection of PSD95 (Cat. No. N3783, dilution 1 : 1000, AP staining) and β-Actin (Synaptic Systems Cat. No. 251 006, dilution 1 : 2000, AP-staining) in wild type (WT) and PSD 95 knock-out (KO) mouse brain homogenates.​

IHC on formalin-fixed paraffin-embedded (FFPE) tissue using the rabbit anti-PSD95 r-hcAb (Cat. No. N3783, dilution 1 μg/ml) reveals the characteristic distribution of PSD95 in coronal sections of mouse hippocampus. All regions of the hippocampus are labeled. However, DAB staining is more intense in the pyramidal cell layer of the CA1 area and the molecular layer of the dentate gyrus (DG). Figure modified from Kilisch et al., 2023 (see References below).

Recombinant anti-PSD95 Antibody (rec. hcAbs)

Cat No: N/A Category:

400,00 

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PSD95 (alternative names: PSD-95 or SAP-90) is a member of the MAGUK family, and it plays an essential role in forming a dynamic scaffold at the post-synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Our sdAb anti-PSD95 is genetically fused to the Fc domain from different species (Rabbit, Mouse, Guinea Pig). Our nanobody binds selectively and strongly to one of the PDZ domains of PSD95, and it does not bind to either of its close family members, PSD93 or SAP102.

Variations:
Conjugation Amount Cat No. RRID
Guinea Pig Fc-fusion 100 µg N3784 AB_3076112
Rabbit Fc-fusion protein 100 µg N3783 AB_3076111
Mouse IgG1 Fc-fusion 100 µg N3782 AB_3076110
Mouse IgG2 Fc-fusion protein 100 µg N3785 AB_3076113
Related Products: -
Clone: 1B2
Host: Alpaca
Produced in: HEK
Application: IF, IHC, WB
Dilution: 1:500 (IF), 10 µg/mL (IHC), 1:1000 WB
Capacity: N/A
Antigen: -
Targets: PSD95
Specificity: Recognizes PSD95 from mouse and rat (other species not tested).
Formulation: Lyophilized from PBS pH 7.4, containing 100 µg rec. hcAbs and 2% BSA (US-Origin). Reconstitute with 100 µL 50% glycerol. We recommend including 0.1 % sodium azide as a preservative if applicable.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols: -
References:
  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (WB-KO validated, ICC/IF, IHC, IHC-P; tested species: rat, mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions. Limited Use Label License: Internal Research Use Only The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.