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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mRuby3 reporter protein with FluoTag®-X2 anti-mRuby3 Alexa Fluor 647 (Cat. No. N3302-AF647-L, dilution 1:500). The mRuby3 signal is represented in red, the corresponding FluoTag® signal is represented in green and the merge of both channels is represented in yellow. Nuclei were visualized by DAPI staining (blue).

 FluoTag-X2 anti-mRuby AF647

PFA-fixed Cos7 cells expressing a TOM70-nfmRuby3 fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mRuby3 coupled to Alexa Fluor 647 (Cat. No. N3302-AF647, dilution 1:500). A Greyscale image of the staining performed with N3302-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

 FluoTag-X2 anti-mRuby AF568

PFA-fixed Cos7 cells expressing a TOM70-nfmRuby3 fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mRuby3 coupled to AZDye 568 (Cat. No. N3302-AF568, dilution 1:500). A Greyscale image of the staining performed with N3302-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

 FluoTag-X2 anti-mRuby At488

PFA-fixed Cos7 cells expressing a TOM70-nfmRuby3 fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mRuby3 coupled to Atto 488 (Cat. No. N3302-At488, dilution 1:500). A Greyscale image of the staining performed with N3302-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.
FluoTag-X2 anti-mRuby AF647
FluoTag-X2 anti-mRuby AF568
FluoTag-X2 anti-mRuby At488

FluoTag®-X2 anti-mRuby3

Cat No: N3302 Category:

420,00 €

FluoTag®-X2 anti-mRuby3 is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes mRuby3, a bright monomeric red fluorescent protein with enhanced brightness and photostability compared to earlier mRuby variants.

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mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor. mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.

An open-source database with the most currently available mRuby variants can be found at the Fluorescent Protein Database FPbase.

Our nanobody binds specifically and strongly to mRuby3. It does not recognize mRuby and mRuby2. For detailed information, please refer to our FP Specificity Chart.

FluoTag®-X2 anti-mRuby3 is site-specifically conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about FluoTags®, please visit our Technology section here.

Haven’t found the perfect fluorophore for your application?
We offer custom conjugation services with most commercially available fluorophores — provided they are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μL N3302-At488-L AB_3076080
AZDye568 200 μL N3302-AF568-L AB_3076078
Atto643 200 μL N3302-At643-L AB_3076081
Alexa647 200 μL N3302-AF647-L AB_3076079
abberior STAR 635P 200 μL N3302-Ab635P-L AB_3076077
Related Products: View related products
Clone: 1F8
Host: Alpaca
Produced in: E.coli
Application:

IF

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: mRUBY3
Specificity: Validated specificity for mRuby3. No reactivity detected with mRuby or mRuby2.
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart.
References:
  1. Zolboot N, Xiao Y, Du JX, et al. MicroRNA mechanisms instructing Purkinje cell specification. Neuron. 2025;113(10):1629-1646.e15. doi:10.1016/j.neuron.2025.03.009 (N3302-AF647)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

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