sdAb anti-mRuby3
Price range: 290,00 € through 655,00 €
sdAb anti-mRuby3 is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes mRuby3, a bright monomeric red fluorescent protein with enhanced brightness and photostability compared to earlier mRuby variants. Available conjugated to Biotin, DBCO, C-terminal Cysteine, or customized upon request. For fluorescent conjugates, see our FluoTag® product line.
mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor. mRuby, mRuby2, and mRuby3 are all excited at 560 nm, with mRuby3 being the brightest.
An open-source database with the most currently available variants can be found at the Fluorescent Protein Database FPbase.
sdAb anti-mRuby3 binds specifically and strongly to mRuby3. It does not recognize mRuby and mRuby2. For detailed information, please refer to our FP Specificity Chart.
| Variations: |
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| Related Products: |
FluoTag®-X2 anti-mRuby3 (Cat. No. N3302) View related products |
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| Clone: | 1F8 | ||||||||||||||||
| Host: | Alpaca | ||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||
| Application: |
EL, IF, IHC Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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| Dilution: | N/A | ||||||||||||||||
| Capacity: | N/A | ||||||||||||||||
| Antigen: | - | ||||||||||||||||
| Targets: | mRUBY3 | ||||||||||||||||
| Specificity: | Validated specificity for mRuby3. No reactivity detected with mRuby or mRuby2. | ||||||||||||||||
| Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
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| kDa: | 15.0 | ||||||||||||||||
| Ext Coef: | 20128 | ||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||
| Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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| Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart. |
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| References: | - | ||||||||||||||||
| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
| Legal terms: |
By purchasing this product you agree to our general terms and conditions. |
