Direct immunofluorescence labeling of a cultured hippocampus neurons. Myelinated neurons were visualized with FluoTag®-X2 anti-CNPase Atto488 (Cat. No. N4102-At488-L; dilution 1:500; green). Nerve-terminals positive for VGLUT1 were visualized with FluoTag®-X2 anti-VGLUT1 AZDye 568 (Cat. No. N1602-AF568-L; dilution 1:500, red). Nuclei were visulized with DAPI (blue).

FluoTag-X2 anti-CNPase AF647

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Alexa Fluor 647 (Cat. No. N4102-AF647, dilution 1:500). A Greyscale image of the staining performed with N4102-AF647. B False color representation of A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

FluoTag-X2 anti-CNPase AF568

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to AZDye 568 (Cat. No. N4102-AF568, dilution 1:500). A Greyscale image of the staining performed with N4102-AF568. B False color representation of A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

FluoTag-X2 anti-CNPase At488

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Atto 488 (Cat. No. N4102-At488, dilution 1:500). A Greyscale image of the staining performed with N4102-At488. B False color representation of A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-X2 anti-CNPase

Cat No: N4102 Category:

400,00 

A fluorophore-conjugated single-domain antibody that specifically recognizes CNPase.

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FluoTag®-X2 anti-CNPase is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes a CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase), also known as CNP1. CNPase is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system. It may also be involved in the RNA metabolism of  myelinating cells of the central nervous system (CNS), the oligodendrocytes.

FluoTag®-X2 anti-CNPase is directly conjugated to two fluorophores per sdAb (FluoTag®X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

 

Variations:
Conjugation Amount Cat No. RRID
AbberiorStar635P 200 μl N4102-Ab635P-L AB_3076122
Alexa647 200 μl N4102-AF647-L AB_3076124
Atto488 200 μl N4102-At488-L AB_3076125
Atto643 200 μl N4102-At643-L AB_3076126
AZDye568 200 μl N4102-AF568-L AB_3076123
Related Products: -
Clone: 1D5
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:500
Capacity: N/A
Antigen: CNPase
Targets: CNPase
Specificity: Recognizes mouse CNPase.
Formulation:

A single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

kDa: 16.4
Ext Coef: 28670
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.