Direct immunofluorescence labeling of a cultured hippocampus neurons. Myelinated neurons were visualized with FluoTag®-X2 anti-CNPase Atto488 (Cat. No. N4102-At488-L; dilution 1:500; green). Nerve-terminals positive for VGLUT1 were visualized with FluoTag®-X2 anti-VGLUT1 AZDye 568 (Cat. No. N1602-AF568-L; dilution 1:500, red). Nuclei were visulized with DAPI (blue).

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Alexa Fluor 647 (Cat. No. N4102-AF647, dilution 1:500). A Greyscale image of the staining performed with N4102-AF647. B False color representation of A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to AZDye 568 (Cat. No. N4102-AF568, dilution 1:500). A Greyscale image of the staining performed with N4102-AF568. B False color representation of A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Atto 488 (Cat. No. N4102-At488, dilution 1:500). A Greyscale image of the staining performed with N4102-At488. B False color representation of A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.