Direct immunofluorescence labeling of a cultured hippocampus neuron. Myelinated neurons were visualized with FluoTag-X2 anti-CNPASe Atto488 (green; 1:500). Nerve-terminals positive for VGLUT1 were visualized with FluoTag-X2 anti-VGLUT1 AZDye 568 (red, 1:500). Nucleii were visulized by dapi (blue).

FluoTag-X2 anti-CNPase AF647

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Alexa Fluor 647 (Cat. No. N4102-AF647, dilution 1:500). A Greyscale image of the staining performed with N4102-AF647. B False color representation of A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

FluoTag-X2 anti-CNPase AF568

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to AZDye 568 (Cat. No. N4102-AF568, dilution 1:500). A Greyscale image of the staining performed with N4102-AF568. B False color representation of A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

FluoTag-X2 anti-CNPase At488

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-CNPase coupled to Atto 488 (Cat. No. N4102-At488, dilution 1:500). A Greyscale image of the staining performed with N4102-At488. B False color representation of A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-X2 anti-CNPase

Cat No: N4102 Category:

400,00 

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CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase) also known as CNP1 is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system. It may also be involved in the RNA metabolism of  myelinating cells of the central nervous system (CNS), the oligodendrocytes.

 

MSDS
Variations:
Conjugation Amount Cat No. RRID
AbberiorStar635P 200 μl N4102-Ab635P-L AB_3076122
Alexa647 200 μl N4102-AF647-L AB_3076124
Atto488 200 μl N4102-At488-L AB_3076125
Atto643 200 μl N4102-At643-L AB_3076126
AZDye568 200 μl N4102-AF568-L AB_3076123
Related Products: -
Clone: 1D5
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:500
Capacity: N/A
Antigen: -
Targets: CNPase
Specificity: Recognizes mouse CNPase.
Formulation: A single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 2.5 µM.
kDa: 16.4
Ext Coef: 28670
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Look at detailed protocols and our specificity chart in our Resource Section.

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.