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Human PBMCs isolated from buffy coat using a Ficoll gradient and co-stained with anti-CD4-APC, anti-CD62L-PE (DREG-56) and our FluoTag®-X2 anti-CD62L-FITC.

 FluoTag-X2 anti-CD62L_AF647

Human PBMCs isolated from buffy coat using a Ficoll gradient and co-stained with a rabbit monoclonal recombinant IgG anti-CD3e, secondary FluoTag®-X2 anti-rabbit IgG At488 (Cat. No. N2402-At488) and FluoTag®-X2 anti-CD62L AF647 (Cat. No. N1402-AF647).

 FluoTag-X2 anti-CD62L_AF568

Human PBMCs isolated from buffy coat using a Ficoll gradient and co-stained with a rabbit monoclonal recombinant IgG anti-CD3e, secondary FluoTag®-X2 anti-rabbit IgG At488 (Cat. No. N2402-At488) and FluoTag®-X2 anti-CD62L AZDye568 (Cat. No. N1402-AF568).

 FluoTag-X2 anti-CD62L_At488

Human PBMCs isolated from buffy coat using a Ficoll gradient and co-stained with a rabbit monoclonal recombinant IgG anti-CD3e, secondary FluoTag®-X2 anti-rabbit IgG AF647 (Cat. No. N2402-AF647) and FluoTag®-X2 anti-CD62L At488 (Cat. No. N1402-At488).
FluoTag-X2 anti-CD62L_AF647
FluoTag-X2 anti-CD62L_AF568
FluoTag-X2 anti-CD62L_At488

FluoTag®-X2 anti-CD62L

Cat No: N1402 Category:

400,00 €

A fluorophore-conjugated single-domain antibody that specifically recognizes the extracellular domain of human lymphocyte marker CD62L (L-selectin).

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FluoTag®-X2 anti-CD62L is derived from an in-house developed single-domain antibody (sdAb) that specifically binds to the human lymphocyte marker CD62L (L-selectin) with high affinity. CD62L is a surface marker protein present on naïve T cells. Its regulation plays a pivotal role in controlling the traffic of T-lymphocytes to and from peripheral lymph nodes. Unlike other T-cell surface marker proteins such as CCR7, which can be found in either central memory cells (Tcm), or in effector memory cells (Tem), CD62L is uniformly expressed in Tcm cells (CD62L+), allowing for a clear differentiation between T-cell populations.

Our nanobody recognizes the extracellular domain of human CD62L.

FluoTag®-X2 anti-CD62L is directly conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) Protocol
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N1402-At488-L AB_3075974
AZDye568 200 μl N1402-AF568-L AB_3075972
Atto643 200 μl N1402-At643-L AB_3075975
Alexa647 200 μl N1402-AF647-L AB_3075973
AbberiorStar635P 200 μl N1402-Ab635P-L AB_3075971
Related Products: -
Clone: P3C11
Host: Alpaca
Produced in: E.coli
Application:

FACS, IF, IHC

  • N1402-At488-L ; N1402-AF647-L; N1402-At643-L; N1402-Ab635P-L are recommended for FACS applications.
  • N1402-AF568-L is not recommended for FACS due to compatibility issues between AZDye568 dye and FACS filters.
  • IF (ICC and IHC) has not yet been tested.
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: CD62L
Specificity: Recognizes the extracellular domain of human CD62L (L-selectin).
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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