Contact Shop

Human PBMCs isolated from buffy coat using a Ficoll gradient
and co-staining with anti-CD4-APC, anti-CD62L-PE (DREG-56)
and our FluoTag-X2 anti-CD62L-FITC.

FluoTag®-X2 anti-CD62L

Cat No: N1402 Category:

375,00 €

FluoTag®-X2 anti-CD62L is derived from an in-house developed single-domain antibody (sdAb) recognizing the human lymphocyte marker CD62L (L-selectin) with high affinity and specificity.

Read more

CD62L, or L-selectin is a surface marker protein present on naïve T cells. Its regulation plays a pivotal role in controlling the traffic of T-lymphocytes to and from peripheral lymph nodes. Compared to other T-cell surface marker proteins, such as CCR7, which can be found in either central memory cells (Tcm), or in effector memory cells (Tem), CD62L is uniformly expressed in Tcm cells (CD62L+), allowing for a clear differentiation between T-cell populations.

FluoTag®-X2 anti-CD62L is derived from an in-house developed single-domain antibody (sdAb) recognizing the human lymphocyte marker CD62L (L-selectin) with high affinity and specificity.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS Protocols
Variations:
Amount Conjugation Cat No.
Atto488 200 μl N1402-At488-L
AZDye568 200 μl N1402-AF568-L
Atto643 200 μl N1402-At643-L
Alexa647 200 μl N1402-AF647-L
AbberiorStar635P 200 μl N1402-Ab635P-L
Related Products: -
Clone: P3C11
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: CD62L
Specificity: Recognizes the extracellular domain of human CD62L (L-selectin).
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Look at detailed protocols in our Resource Section.

N1402-At488-L ; N1402-AF647-L; N1402-At643-L N1402-Ab635P-L are recommended for FACS

N1402-AF568-L is not recommended for FACS, due to compatibility issues of AZDye568 dye and FACS filters

ICC not tested for CD62L
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

Related products