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PFA fixed SARS-CoV-2 infected cells stained with FluoTag-Q anti-SARS-CoV-2 Spike protein S1 SC3 (red) (cat. no. N3501) and mouse anti-nucleocapsid (green)(Synaptic Systems, cat. No. HS-452 011). Nuclei were visualized by DAPI staining (blue). Courtesy of Dr. Marco van Ham, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.

FluoTag®-Q anti-SARS-CoV-2 Spike protein S1

Cat No: N3501 Category:

400,00 €

The FluoTag-Q anti-SARS-CoV-2 S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.

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This anti-Sars-CoV-2 Spike protein S1 nanobody was developed by the team of Alejandro Rojas-Fernandez at the Institute of Medicine at the Universidad Austral de Chile. We acquired the exclusive license to produce this nanobody and make it available for research and development purposes. The FluoTag-Q anti-SARS-CoV-2 S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N3501-At488-L AB_3076088
AZDye568 200 μl N3501-AF568-L AB_3076086
Atto643 200 μl N3501-At643-L AB_3076089
Alexa647 200 μl N3501-AF647-L AB_3076087
AbberiorStar635P 200 μl N3501-Ab635P-L AB_3076085
Related Products: -
Clone: W25
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:1000 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: SARS-CoV-2 S1
Specificity: Recognizes the RBD of the SARS-CoV-2 S1 protein.
Formulation: A single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Look at detailed protocols and our specificity chart in our Resource Section.
References:
  1. Valenzuela Nieto G, Jara R, Watterson D, et al. Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody. Sci Rep. 2021;11(1):3318. Published 2021 Feb 8. doi:10.1038/s41598-021-82833-w
  2. Rong Z. Unraveling proteomic changes of heterogeneous SARS-CoV-2 infection identified by panoptic imaging. PhD thesis (2023), Faculty of Medicine, LMU München. (IHC; human kidney)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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