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PFA fixed SARS-CoV-2 infected cells stained with FluoTag®-Q anti-SARS-CoV-2 Spike protein S1 SC3 (red) (Cat. No. N3501) and mouse anti-nucleocapsid (green)(Synaptic Systems, Cat. No. HS-452 011). Nuclei were visualized by DAPI staining (blue). Image courtesy of Dr. Marco van Ham, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.

FluoTag®-Q anti-SARS-CoV-2 Spike protein S1

Cat No: N3501 Category:

400,00 €

The FluoTag®-Q anti-SARS-CoV-2 S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.

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FluoTag®-Q anti-SARS-CoV-2 Spike protein S1 is derived from nanobody that was developed by the team of Alejandro Rojas-Fernandez at the Institute of Medicine at the Universidad Austral de Chile. We acquired the exclusive license to produce this nanobody and make it available for research and development purposes. The FluoTag®-Q anti-SARS-CoV-2 Spike protein S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.

FluoTag®-Q anti-SARS-CoV-2 Spike protein S1 is directly conjugated to one fluorophore per sdAb (FluoTag®-Q variant), which makes it ideal for more quantitative readouts. To learn more about the FluoTags®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) Protocol
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N3501-At488-L AB_3076088
AZDye568 200 μl N3501-AF568-L AB_3076086
Atto643 200 μl N3501-At643-L AB_3076089
Alexa647 200 μl N3501-AF647-L AB_3076087
AbberiorStar635P 200 μl N3501-Ab635P-L AB_3076085
Related Products:

sdAb anti-SARS-CoV-2 Spike protein S1 (Cat. No. N3505)

Recombinant anti-SARS-CoV-2 Spike protein S1 antibody (rHcAb) (Cat. No. N3583)

sdAb anti-SARS-CoV-2 Nucleocapsid (Cat. No. N3605)

FluoTag®-Q anti-SARS-CoV-2 Nucleocapsid (Cat. No. N3601)
Clone: W25
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:1000 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: SARS-CoV-2 S1
Specificity: Recognizes the RBD of the SARS-CoV-2 S1 protein.
Formulation:

A single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Valenzuela Nieto G, Jara R, Watterson D, et al. Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody. Sci Rep. 2021;11(1):3318. Published 2021 Feb 8. doi:10.1038/s41598-021-82833-w (Nanobody W25 clone development and characterization)
  2. Rong Z, Mai H, Ebert G, et al. Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19. Cell Host Microbe. 2024;32(12):2112-2130.e10. doi:10.1016/j.chom.2024.11.007 (IF; whole mouse body labeling)
  3. Rong Z. Unraveling proteomic changes of heterogeneous SARS-CoV-2 infection identified by panoptic imaging. PhD thesis (2023), Faculty of Medicine, LMU München. (IHC; human kidney)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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