FluoTag®-Q anti-SARS-CoV-2 Nucleocapsid

Cat No: N3601 Category:

375,00 

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The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.

Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS
Variations:
Conjugation Amount Cat No.
Alexa647 200 μl N3601-AF647-L
Atto488 200 μl N3601-At488-L
AZDye568 200 μl N3601-AF568-L
Related Products: -
Clone: 2A7
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500
Capacity: N/A
Antigen: SARS-CoV-2 Nucleocapsid
Specificity: Recognizes the Nucleocapsid protein of SARS-CoV-2 and SARS-CoV-1.
Formulation: A single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Look at detailed protocols and our specificity chart in our Resource Section.

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.