The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.
Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.
| Variations: |
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| Related Products: | - | ||||||||||||||||||
| Clone: | 2A7 | ||||||||||||||||||
| Host: | Alpaca | ||||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||||
| Application: | IF | ||||||||||||||||||
| Dilution: | 1:500 | ||||||||||||||||||
| Capacity: | N/A | ||||||||||||||||||
| Antigen: | - | ||||||||||||||||||
| Targets: | SARS-CoV-2 Nucleocapsid | ||||||||||||||||||
| Specificity: | Recognizes the Nucleocapsid protein of SARS-CoV-2 and SARS-CoV-1. | ||||||||||||||||||
| Formulation: | A single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM | ||||||||||||||||||
| kDa: | - | ||||||||||||||||||
| Ext Coef: | - | ||||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||||
| Storing: | Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C. | ||||||||||||||||||
| Protocols: |
Look at detailed protocols and our specificity chart in our Resource Section. |
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| References: | - | ||||||||||||||||||
| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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