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COS-7 cells transfected with ALFA-TOM70, or Vimentin-ALFA were stained with the polyclonal rabbit anti-ALFA antibody (Cat. no. N1581, dilution 1:500) and FluoTag-X4 anti-rabbit IgG Abberior 580 (cat. no N2404-Ab580-S). Nuclei were visualized by DAPI staining.

Affinity purified rabbit anti-ALFA polyclonal antibody

Cat No: N1581 Category:

400,00 €

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The anti-ALFA polyclonal antibody raised in rabbits recognizes the ALFA-tag (SRLEEELRRRLTE).

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
- 50 µg N1581 AB_3075997
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Clone: Affinity purified polyclonal rabbit IgG antibody
Host: Rabbit
Produced in: Rabbit
Application: IF, IHC, WB
Dilution: 1:500
Capacity: N/A
Antigen: -
Targets: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE).
Formulation:

50 µg affinity purfied antibody lyophilized from PBS. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water to get a 0.5 mg/mL stock. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized antibodies can be stored at 2-8°C for up to 12 months. After reconstitution, working aliquots can be stored at 4°C for up to 4 weeks. For long term storage (up to 6 months), store reconstituted antibodies at -20°C or below. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

You can find protocols on our Resources page.
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. Reinders NR, van der Spek SJF, Klaassen RV, et al. Amyloid-β-Driven Synaptic Deficits Are Mediated by Synaptic Removal of GluA3-Containing AMPA Receptors. J Neurosci. 2025;45(9):e0393242024. Published 2025 Feb 26. doi:10.1523/JNEUROSCI.0393-24.2024  (IF; rat hippocampal neurons)
  3. da Silva Pescador G, Baia Amaral D, Varberg JM, et al. Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis. Cell Rep. 2024;43(10):114769. doi:10.1016/j.celrep.2024.114769 (WB; zebrafish)
  4. Baldinotti R, Pauzin FP, Fevang H, Ishizuka Y, Bramham CR. A Nanobody-Based Proximity Ligation Assay Detects Constitutive and Stimulus-Regulated Native Arc/Arg3.1 Oligomers in Hippocampal Neuronal Dendrites. Mol Neurobiol. Published online October 5, 2024. doi:10.1007/s12035-024-04508-7 (Proximity ligation assay /PLA; HEK293FT cells and hippocampal neurons)
  5. Bellec M, Chen R, Dhayni J, et al. Boosting the toolbox for live imaging of translation. RNA. 2024;30(10):1374-1394. Published 2024 Sep 16. doi:10.1261/rna.080140.124 (immuno-smFISH; fruit fly)
  6. Körner C, Schäfer JH, Esch BM, et al. The structure of the Orm2-containing serine palmitoyltransferase complex reveals distinct inhibitory potentials of yeast Orm proteins. Cell Rep. 2024;43(8):114627. doi:10.1016/j.celrep.2024.114627 (WB; yeast)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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