Affinity purified rabbit anti-ALFA polyclonal antibody

Cat No: N1581 Category:

400,00 

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The anti-ALFA polyclonal antibody raised in rabbits recognizes the ALFA-tag (SRLEEELRRRLTE).

MSDS
Variations:
Conjugation Amount Cat No. RRID
- 50 µg N1581 AB_3075997
Related Products: -
Clone: Affinity purified polyclonal rabbit IgG antibody
Host: Rabbit
Produced in: Rabbit
Application: IF, IHC, WB
Dilution: 1:500
Capacity: N/A
Antigen: -
Targets: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE).
Formulation:

50 µg affinity purfied antibody lyophilized from PBS. Use 100 µL pure water for reconstitution to get a 0.5 mg/mL stock. We recommend including 0.1 % sodium azide as a preservative if applicable.

kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing:

Vials containing lyophilized antibodies can be stored at 4°C for up to 6 months. Vials containing reconstituted antibodies should be stored at -20°C or below.

Protocols:

You can find protocols on our Resources page.

References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. Baldinotti R, Pauzin FP, Fevang H, Ishizuka Y, Bramham CR. A Nanobody-Based Proximity Ligation Assay Detects Constitutive and Stimulus-Regulated Native Arc/Arg3.1 Oligomers in Hippocampal Neuronal Dendrites. Mol Neurobiol. Published online October 5, 2024. doi:10.1007/s12035-024-04508-7 (Proximity ligation assay /PLA; HEK293FT cells and hippocampal neurons)
  3. Bellec M, Chen R, Dhayni J, et al. Boosting the toolbox for live imaging of translation. RNA. 2024;30(10):1374-1394. Published 2024 Sep 16. doi:10.1261/rna.080140.124 (immuno-smFISH; fruit fly)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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