Ubiquitin K63 Selector

Cat No: N1910 Category:


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Proteins in cells can be ubiquitinated where E3 ligase catalyzes the last step of this process. After conjugating the initial ubiquitin on the target protein, further ubiquitin proteins can be conjugated to the first ubiquitin resulting in a polyubiquitin chain. The conjugation of new ubiquitins to form a poly-chain happens at one of the seven lysines (K) present in ubiquitin. The addition of a new ubiquitin on the lysine at position 63 (K63) provides a different fate to the target protein than if linked on another K. (change!)

Our sdAb binds most specifically to this linkage, thus resulting in the exclusive recognition of K63 linkages on polyubiquitin chains.

This product was a co-development with

Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N1910 AB_3076017
Related Products: -
Clone: D1-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 2.5 µg K-63 linked Ubiquitin per µL of packed beads
Antigen: -
Targets: Ubiquitin K63 linkage
Specificity: Recognizes K63-linkage of polyubiquitins
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4 °C for upto 12 months. Do not freeze!
Protocols: -
  1. Gonzalez-Santamarta M, Ceccato L, Carvalho AS, Rain JC, Matthiesen R, Rodriguez MS. Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies. Methods Mol Biol. 2023;2602:125-136. doi:10.1007/978-1-0716-2859-1_9 (Isolation of ubiquitylated proteins, MS; MCL cells)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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