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Ubiquitin K63 Selector

Cat No: N1910 Categories: ,

290,00 €

Ubiquitin K63 Selector is based on a high-affinity single-domain antibody (sdAb), which exclusively recognizes K63 linkages in polyubiquitin chains and is covalently immobilized on 4% cross-linked agarose beads.

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Ubiquitination is a post-translational modification in which an E3 ligase catalyzes the attachment of ubiquitin to a substrate protein. After the initial ubiquitin is conjugated, additional ubiquitin molecules can be linked through one of the seven lysine (K) residues in ubiquitin to form polyubiquitin chains. K63-linked chains are primarily associated with non-proteasomal functions, such as intracellular signaling, DNA repair, and endocytosis.

Ubiquitin K63 Selector is based on a single-domain antibody (sdAb), covalently immobilized on agarose beads, that specifically recognizes K63-linked polyubiquitin chains. It can be used in immunoprecipitation for the selective enrichment of K63-ubiquitinated proteins.

This product was a co-development with

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 2 mL slurry N1910 AB_3076017
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Clone: D1-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 2.5 µg K-63 linked Ubiquitin per µL of packed beads
Antigen: -
Targets: Ubiquitin K63 linkage
Specificity: Recognizes K63-linkage of polyubiquitins
Formulation:

50% slurry in PBS containing 20% Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Store at 4°C for up to 12 months. Do not freeze!
Protocols: -
References:
  1. Weyer Y, Schwabl SI, Tang X, et al. The Dsc ubiquitin ligase complex identifies transmembrane degrons to degrade orphaned proteins at the Golgi. Nat Commun. 2024;15(1):9257. Published 2024 Oct 26. doi:10.1038/s41467-024-53676-6 (IP; yeast)
  2. Gonzalez-Santamarta M, Ceccato L, Carvalho AS, Rain JC, Matthiesen R, Rodriguez MS. Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies. Methods Mol Biol. 2023;2602:125-136. doi:10.1007/978-1-0716-2859-1_9 (Isolation of ubiquitylated proteins, MS; MCL cells)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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