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Cartoon representation of ubiquitin-protein, highlighting the secondary structure. α-helices are coloured in blue and β-strands in green. The sidechains of the 7 lysine residues are indicated by orange sticks. The two best-characterised attachment points for further ubiquitin molecules in polyubiquitin chain formation (lysines 48 & 63) are labelled. The image was created using PyMOL from PDB id 1ubi. Image adapted from Rogerdodd under CC BY-SA 3.0

sdAb anti-Ubiquitin K48

Cat No: N1805 Category:

275,00 € – 625,00 €

sdAb anti-Ubiquitin K48  is a high-affinity single-domain antibody (sdAb) recognizing the K48 linkage in ubiquitin.

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Proteins in cells can be ubiquitinated where E3 ligase catalyzes the last step of this process. After conjugating the initial ubiquitin on the target protein, further ubiquitin proteins can be conjugated to the first ubiquitin resulting in a polyubiquitin chain. The conjugation of new ubiquitins to form a poly-chain happens at one of the seven lysines (K) present in ubiquitin. The addition of a new ubiquitin on the lysine at position 48 (K48) is a signal for the target protein to be sent to the proteasome for degradation.

Our sdAb binds most specifically to this K48 linkage on polyubiquitin chains.

This product was a co-development with

 

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N1805-250ug AB_3076010
DBCO 250 μg N1805-DBCO AB_3076012
Biotin 250 μg N1805-Biotin AB_3076011
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Clone: A4-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Ubiquitin K48 linkage
Specificity: Recognizes K48-linkages of polyubiquitin.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 15,2
Ext Coef: 30098
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.