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Cartoon representation of ubiquitin-protein, highlighting the secondary structure. α-helices are coloured in blue and β-strands in green. The sidechains of the 7 lysine residues are indicated by orange sticks. The two best-characterised attachment points for further ubiquitin molecules in polyubiquitin chain formation (lysines 48 & 63) are labelled. The image was created using PyMOL from PDB id 1ubi. Image adapted from Rogerdodd under CC BY-SA 3.0

sdAb anti-Ubiquitin K48

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250,00 € – 600,00 €

sdAb anti-Ubiquitin K48  is a high-affinity single-domain antibody (sdAb) recognizing the K48 linkage in ubiquitin.

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Proteins in cells can be ubiquitinated where E3 ligase catalyzes the last step of this process. After conjugating the initial ubiquitin on the target protein, further ubiquitin proteins can be conjugated to the first ubiquitin resulting in a polyubiquitin chain. The conjugation of new ubiquitins to form a poly-chain happens at one of the seven lysines (K) present in ubiquitin. The addition of a new ubiquitin on the lysine at position 48 (K48) is a signal for the target protein to be sent to the proteasome for degradation.

Our sdAb binds most specifically to this K48 linkage on polyubiquitin chains.

This product was a co-development with

 

MSDS
Variations:
Conjugation Amount Cat No.
DBCO 250 μg N1805-DBCO
unconjugated C-terminal Cys 250 μg N1805-250µg
Biotin 250 μg N1805-Biotin
Related Products: -
Clone: A4-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: N/A
Antigen: Ubiquitin K48 linkage
Specificity: Recognizes K48-linkages of polyubiquitin.
Formulation: DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.
kDa: 15,2
Ext Coef: 30098
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols: -
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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