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sdAb anti-TagFP

Cat No: N0505 Category:

250,00 € – 600,00 €

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TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2. (change!!!!)

Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2. Look at our specificity chart in the resource section.

MSDS Protocols
Variations:
Amount Conjugation Cat No.
unconjugated C-terminal Cys 250 μg N0505-250µg
Biotin 250 μg N0505-Biotin
DBCO 250 μg N0505-DBCO
Related Products: -
Clone: 1H7
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: tagBFP
Specificity: Recognizes TagRFP, Tag BFP and mTagBFP2.
Formulation: DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water for immidate use (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs).
kDa: 16.0
Ext Coef: 20128
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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