TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2. (change!!!!)
Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2. Look at our specificity chart in the resource section.
Variations: |
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Related Products: | - | ||||||||||||
Clone: | 1H7 | ||||||||||||
Host: | Alpaca | ||||||||||||
Produced in: | E.coli | ||||||||||||
Application: | IF, IHC | ||||||||||||
Dilution: | N/A | ||||||||||||
Capacity: | N/A | ||||||||||||
Antigen: | - | ||||||||||||
Targets: | tagBFP | ||||||||||||
Specificity: | Recognizes TagRFP, Tag BFP and mTagBFP2. | ||||||||||||
Formulation: | DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water for immidate use (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). | ||||||||||||
kDa: | 16.0 | ||||||||||||
Ext Coef: | 20128 | ||||||||||||
Shipping: | Ambient temperature | ||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | ||||||||||||
Protocols: |
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. |
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References: | - | ||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |