TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2. (change!!!!)
Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2. Look at our specificity chart in the resource section.
| Variations: |
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| Related Products: | - | ||||||||||||||||
| Clone: | 1H7 | ||||||||||||||||
| Host: | Alpaca | ||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||
| Application: | IF, IHC | ||||||||||||||||
| Dilution: | N/A | ||||||||||||||||
| Capacity: | N/A | ||||||||||||||||
| Antigen: | - | ||||||||||||||||
| Targets: | tagBFP | ||||||||||||||||
| Specificity: | Recognizes TagRFP, Tag BFP and mTagBFP2. | ||||||||||||||||
| Formulation: | DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water for immidate use (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). | ||||||||||||||||
| kDa: | 16.0 | ||||||||||||||||
| Ext Coef: | 20128 | ||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||
| Storing: | Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | ||||||||||||||||
| Protocols: |
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. |
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| References: |
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| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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