sdAb anti-TagFP

Cat No: N0505 Category:

275,00 625,00 

asAb anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2.

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Fluorescent proteins (FPs), widely used as protein tags in cell biology and microscopy, have revolutionized protein imaging and biochemical applications. TagRFP, a monomeric red fluorescent protein, was engineered from TurboRFP (derived from Entacmaea quadricolor) to exhibit an extended fluorescence lifetime and enhanced photostability (Merzlyak et al., 2007). Through further mutagenesis, this red-emitting protein was transformed into the blue-emitting TagBFP (Subach et al., 2008). A few years later, an improved version known as mTagBFP2 was introduced, offering greater brightness and stability (Subach et al., 2011). In 2007, mKate was developed from TagRFP, noted for its excellent far-red fluorescence and photostability (Shcherbo et al., 2007). The most currently available variants can be found on an open-source Fluorescent Protein Database FPbase.

sdAb anti-TagFP binds strongly to TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. For detailed information, please refer to our FP Specificity Chart.

MSDS
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N0505-250ug AB_3075939
Biotin 250 μg N0505-Biotin AB_3075940
DBCO 250 μg N0505-DBCO AB_3075941
Related Products: -
Clone: 1H7
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: TagFP
Specificity: Recognizes TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2.
Formulation: DBCO- and biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0, 150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µl of pure water for immediate use (unconjugated sdAbs), or with 250 µl of 50% glycerol in deionized water for storage at -80°C (DBCO- and biotin-conjugated sdAbs).
kDa: 16.0
Ext Coef: 20128
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

These products are not recommended for detecting proteins in Western blot, as sdAbs primarily recognize native or folded proteins.

For more details check our Protocols in Resource Section and FP Specificity Chart.

References:
  1. Hellmeier J, Strauss S, Xu S, et al. Quantification of absolute labeling efficiency at the single-protein level. Nat Methods. Published online April 24, 2024. doi:10.1038/s41592-024-02242-5 (ICC/IF; DNA-PAINT)
  2. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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