Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mRuby reporter protein with FluoTag®-X2 anti-mRuby Atto 488 (Cat. No. N3302-At488-L, dilution 1:500). Nuclei were visualized by DAPI staining (blue).
250,00 € – 600,00 €
sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricol0r.
mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.
Our nanobody binds specifically and strongly to mRuby fluorescent proteins. Have a look at our specificity chart in the resource section.
|Application:||ELISA, IF, IHC|
|Specificity:||Recognizes most common mRuby derivatives.|
|Formulation:||DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.|
|Storing:||Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
You can find protocols on our Resources page
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|