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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mRuby reporter protein with FluoTag®-X2 anti-mRuby Atto 488 (Cat. No. N3302-At488-L, dilution 1:500). Nuclei were visualized by DAPI staining (blue).

sdAb anti-mRuby

Cat No: N3305 Category:

Price range: 290,00 € through 655,00 €

sdAb anti-mRuby is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes mRuby and its derivatives. mRuby is a bright monomeric red fluorescent protein originally derived from Entacmaea quadricolor.

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mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor. mRuby, mRuby2, and mRuby3 are all excited at 560 nm, with mRuby3 being the brightest.

sdAb anti-mRuby binds specifically and strongly to all three mRuby variants: mRuby, mRuby2, and mRuby3. For detailed information, please refer to our FP Specificity Chart.

MSDS
sdAb unconjugated
sdAb-Biotin/DBCO
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 ÎĽg N3305-250ug AB_3076082
Biotin 250 ÎĽg N3305-Biotin AB_3076083
DBCO 250 ÎĽg N3305-DBCO AB_3076084
Related Products:

FluoTag®-X2 anti-mRuby (Cat. No. N3302)

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Clone: 1F8
Host: Alpaca
Produced in: E.coli
Application:

EL, IF, IHC

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mRUBY3
Specificity: Recognizes most common mRuby derivatives. For more deatilas check our Specificity Chart.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 15.0
Ext Coef: 20128
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

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