275,00 € – 625,00 €
sdAb anti-mRuby is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes mRuby and its derivatives. mRuby is a bright monomeric red fluorescent protein originally derived from Entacmaea quadricolor.
mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor. mRuby, mRuby2, and mRuby3 are all excited at 560 nm, with mRuby3 being the brightest.
sdAb anti-mRuby binds specifically and strongly to all three mRuby variants: mRuby, mRuby2, and mRuby3. Refer to our Specificity Chart
Variations: |
|
||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related Products: | |||||||||||||||||
Clone: | 1F8 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: |
EL, IF, IHC Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
||||||||||||||||
Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | - | ||||||||||||||||
Targets: | mRUBY3 | ||||||||||||||||
Specificity: | Recognizes most common mRuby derivatives. For more deatilas check our Specificity Chart. | ||||||||||||||||
Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
||||||||||||||||
kDa: | 15.0 | ||||||||||||||||
Ext Coef: | 20128 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
||||||||||||||||
Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page. |
||||||||||||||||
References: | - | ||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |