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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mRuby reporter protein with FluoTag®-X2 anti-mRuby Atto 488 (Cat. No. N3302-At488-L, dilution 1:500). Nuclei were visualized by DAPI staining (blue).

sdAb anti-mRuby

Cat No: N3305 Category:

250,00 € – 600,00 €

sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricol0r.

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mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.

Our nanobody binds specifically and strongly to mRuby fluorescent proteins. Have a look at our specificity chart in the resource section.

 

MSDS Protocols
Variations:
Amount Conjugation Cat No.
unconjugated C-terminal Cys 250 μg N3305-250µg
Biotin 250 μg N3305-Biotin
DBCO 250 μg N3305-DBCO
Related Products: -
Clone: 1F8
Host: Alpaca
Produced in: E.coli
Application: ELISA, IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mRUBY3
Specificity: Recognizes most common mRuby derivatives.
Formulation: DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.
kDa: 15.0
Ext Coef: 20128
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart

You can find protocols on our Resources page
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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