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Immunostaining of PFA fixed rat hippocampus neurons with FluoTag®-X2 anti-Syt1 Cytosolic Domain AF568 (Cat. No. N4302-AZ568-L, dilution 1:500). The confocal image is shown on the left side and the corresponding STED image is shown on the right side.

FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51)

Cat No: N4302 Category:

420,00 €

A fluorophore-conjugated single-domain antibody that specifically recognizes the cytoplasmic domain of murine and rat Synaptotagmin 1 (Syt1).

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FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) is derived from a single-domain antibody (sdAb) that specifically recognizes a synaptotagmin 1 (Syt1), a member of a protein family consisting of 17 members. Synaptotagmins are single-pass transmembrane proteins containing two calcium-binding domains in their intracellular portion. Synaptotagmin 1 is enriched at the presynaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase in calcium concentration.

Our nanobody strongly and specifically binds to the cytosolic portion of mouse and rat Syt1 (other species not tested).

FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) is directly conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

Note: We offer two anti-Synaptotagmin 1 Cytosolic Domain sdAb clones, #A51 and #7D5, with comparably high specificity and performance. Clone selection can be based on availability or preference for published references (see References section below). Full characterization data have been published for clone #A51 here.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) Protocol
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μL N4302-At488-L AB_3076138
Alexa Fluor™ 568 200 μL N4302-AF568-L AB_3076136
abberior STAR 635P 200 μL N4302-Ab635P-L AB_3076135
Atto643 200 μL N4302-At643-L AB_3076139
Alexa Fluor™ 647 200 μL N4302-AF647-L AB_3076137
Janelia Fluor 635b 200 μL N4302-JF635B-L -
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sdAb anti-Syt1 Cytosoli Domain (A51) (Cat. No. N4305)

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Clone: A51
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic C2A domain of rat, mouse and by homology also human Synaptotagmin 1 (other species not tested).
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Unterauer EM, Schentarra EM, Jevdokimenko K, et al. Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons. STAR Protoc. 2025;6(1):103637. doi:10.1016/j.xpro.2025.103637 (ICC/IF; DNA-PAINT)
  2. Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
  3. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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