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N4802_FluoTag®-X2 anti-Syt1 Luminal Domain AZDye568_NanoTag

Mature rat hippocampal neurons (14 DIV) were incubated live at 37°C & 5% CO₂ in full Neurobasal medium with 100 nM FluoTag®-X2 anti-Syt1 Luminal Domain AZDye568 (Cat. No. N4802-AF568-L, dilution 1:25) for 30 min. After washing and PFA fixation, cells were counterstained with FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) directly conjugated to Atto488 (Cat. No. N4302-AT488-L, dilution 1:500). Images were acquired using a 60× oil-immersion objective on an epifluorescence microscope. The FluoTag®-X2 anti-Syt1 Luminal Domain labels only active/recycling synapses (A, magenta), whereas FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) labels the total Syt1 pool (B, green). Arrowheads indicate expected colocalization at some synapses. Scale bar: 20 µm.

 N4802_FluoTag®-X2 anti-Syt1 Luminal Domain Alexa647_NanoTag

Mature rat hippocampal neurons (14 DIV) were incubated live at 37°C & 5% CO₂ in full Neurobasal medium 100 nM FluoTag®-X2 anti-Syt1 Luminal Domain Alexa647 (Cat. No. N4802-AF647-L, dilution 1:25) for 30 min. After washing and PFA fixation, cells were counterstained with FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) directly conjugated to Atto488 (Cat. No. N4302-AT488-L, dilution 1:500). Images were acquired using a 60× oil-immersion objective on an epifluorescence microscope. The FluoTag®-X2 anti-Syt1 Luminal Domain labels only active/recycling synapses (A, red), whereas FluoTag®-X2 anti-Syt1 Cytosolic Domain (A51) labels the total Syt1 pool (B, green). Arrowheads indicate expected colocalization at some synapses. Scale bar: 20 µm.
N4802_FluoTag®-X2 anti-Syt1 Luminal Domain AZDye568_NanoTag
N4802_FluoTag®-X2 anti-Syt1 Luminal Domain Alexa647_NanoTag

FluoTag®-X2 anti-Syt1 Luminal Domain

Cat No: N4802 Category:

420,00 €

A fluorophore-conjugated single-domain antibody specifically recognizing the luminal/extracellular domain of Synaptotagmin 1 (Syt1). This reagent is intended exclusively for use in living neurons to label active synapses that recycle Syt1 following synaptic vesicle fusion.

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FluoTag®-X2 anti-Syt1 Luminal Domain is derived from a single-domain antibody (sdAb) that specifically recognizes the luminal (or extracellular) domain of Synaptotagmin 1 (Syt1). Syt1 belongs to the synaptotagmin protein family, which in mammals comprises 17 single-pass transmembrane proteins containing two intracellular calcium-binding domains. Synaptotagmin 1 is enriched at presynaptic boutons on axon terminals, where it is associated with synaptic vesicles, and implicated in triggering vesicle fusion and neurotransmitter release upon a local increase in calcium concentration.

Our sdAb anti-Syt1 Luminal Domain binds specifically to the mouse and rat Syt1 luminal/extracellular epitope KEDAFSKLKQKF, and the human epitope KEDAFSKLKEKF (other species not tested).

FluoTag®-X2 anti-Syt1 Luminal Domain is directly conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about available FluoTag® variants, please visit our Technology section here.

Important Note: The reagent is intended exclusively for use in live-cell systems to label active synapses. Aldehyde-based fixatives (including paraformaldehyde, glutaraldehyde, glyoxal, or related compounds) modify lysines within the target epitope, thereby significantly impairing Syt1 recognition.

Comprehensive characterization data for this sdAb anti-Syt1 Luminal Domain have been published here, where it is referred to as NbLumSyt1.

sdAb anti-Syt1 Luminal Domain (clone #1F12) binds to the extracellular/luminal domain of Syt1 in non-fixed samples. We also offer reagents based on sdAbs anti-Syt1 Cytosolic Domain (clones #A51 and #7D5), including labeled sdAbs, FluoTags®-X2, and recombinant heavy-chain antibodies (rHcAbs). These reagents specifically recognize the cytosolic portion of Syt1 in fixed and non-fixed samples, thereby enabling detection of the total Syt1 pool. View the reagents targeting the Syt1 cytosolic domain here.

MSDS
Protocols
Reconstitution and Storage
Syt1 Lum Labeling Manual
Variations:
Conjugation Amount Cat No. RRID
Alexa Fluor™ 568 200 μL N4802-AF568-L -
Alexa Fluor™ 647 200 μL N4802-AF647-L -
Related Products: View related products
Clone: 1F12
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:25 (corresponding to 100 nM final concentration)
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (luminal)
Specificity: Recognizes the non-fixed extracellular or luminal domain of mouse and rat Synaptotagmin 1 (epitope KEDAFSKLKQKF) and human Synaptotagmin 1 (epitope KEDAFSKLKEKF; Q75E substitution). Other species not tested.
Formulation:

A fluorescently labeled sdAb clone lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 200 µL of sterile 50% glycerol (v/v) in deionized water. When reconstituted with 200 µL, the concentration of sdAb is 2.5 µM (5 µM fluorophore). For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 15.0 kDa + dyes
Ext Coef: 27180
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Goel R, Jevdokimenko K, Rehm R, et al. A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons. J Nanobiotechnology. 2026;24(1):443. Published 2026 May 14. doi:10.1186/s12951-026-04489-w
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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