New FluoTag®-X4 anti-GFP abberior STAR variants
Optimal solution for detecting GFP-tagged proteins with STED
Our well-established FluoTag®-X4 anti-GFP is now available conjugated to abberior STAR RED, ORANGE, GREEN, and 460L, offering exceptional brightness, photostability, and localization precision—perfect for high-resolution imaging.
sdAb precision & abberior STAR brilliance – A perfect STED pair
Our FluoTag®-X4 anti-GFP binds strongly to most fluorescent proteins derived from Aequorea victoria, enabling direct immunofluorescence (IF) without the need for a secondary antibody. Thanks to its small size (~3 nm; 15 kDa), the FluoTag®-X4 achieves deep sample penetration, making it highly effective in dense cellular environments and thick specimens, all while minimizing artifacts in IF.
The FluoTag®-X4 anti-GFP is a blend of two high affinity monovalent single-domain antibody (sdAb) clones, each binding to distinct epitopes on GFP. Every sdAb carries two fluorophores, decorating GFP-tagged proteins with a total of four fluorophores for enhanced signal intensity.
The abberior STAR fluorescent dyes were design by abberior experts to meet the specific requirements of the Stimulated Emission Depletion (STED) microscopy. They cover a broad spectral range from blue (432 nm) to red (638 nm), with dyes specifically optimized for 775 nm STED (STAR RED) and provide bright, sharp, and background-free staining, ensuring exceptional imaging quality.
FluoTag®-X4 anti-GFP variants conjugated to abberior STAR dyes available now:
- FluoTag®-X4 anti-GFP abberior STAR 460L (Cat. No. N0304-Ab460L-L)
- FluoTag®-X4 anti-GFP abberior STAR GREEN (Cat. No. N0304-AbGREEN-L)
- FluoTag®-X4 anti-GFP abberior STAR ORANGE (Cat. No. N0304-AbORANGE-L)
- FluoTag®-X4 anti-GFP abberior STAR RED (Cat. No. N0304-AbRED-L)
Why choose FluoTag®-X4 anti-GFP with abberior STAR dyes?
- Exceptional Photostability: Perfect for STED, 2-photon microscopy, and long-term imaging.
- Superior Brightness: Faster acquisition and strong signals, even for low-abundance targets.
- Broad Spectral Range (432-638 nm): Multiplex imaging with standard laser lines & filter sets.
- Low Background Staining: Hydrophilic dyes for clear imaging in cells & tissues.
- Proven Research Performance: Widely used and recognized in leading microscopy studies worldwide.
What abberior experts say
“FluoTag®-X4 anti-GFP, together with our STED-optimized abberior STAR dyes, is a dream team! The bright and photostable dyes, combined with a highly specific, small nanobody, enable outstanding resolution for detecting GFP-tagged targets—completely background-free.”
— Kristina Liebe, R&D Scientist, abberior“By choosing these new fluorescent anti-GFP nanobodies, you get the best of all worlds: minimal label size for nanoscopy, outstanding brightness from STED-optimized abberior STAR dyes, and quantitative, high-affinity binding from FluoTag®-X4 anti-GFP, a blend of two monoclonal nanobodies developed by NanoTag Biotechnologies.”
— Christof Hepp, R&D Scientist, abberior“The collaboration between NanoTag Biotechnologies and abberior was excellent. Communication was always a pleasure. This is clearly reflected in the outstanding final product: FluoTag®-X4 anti-GFP, together with our STED-optimized abberior STAR dyes—delivering exceptional performance, superior resolution, and reliable results.”
— Christian Wurm, Head of Application, abberiorExplore all FluoTag®-X4 anti-GFP abberior STAR conjugates!