A, Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP
B, Immunoprecipitation of mCherry (•) from HeLa lysate.
In/Ft: 1/1000 of input and non-bound material.
E: Eluate from 1 µl of beads
* Specific maturation band from RFP family members (see e.g. Gross et al. PNAS (2000) 11990-11995)
C, Control experiment using functionalized beads lacking sdAbs.
RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector thus features high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background. RFP Selector immobilizes a wide range of RFP derivatives including mCherry and mScarlet. For details see our Product Specificity Chart below. RFP Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). RFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet. Does not cross-react with GFP or mTagBFP/mTagRFP derivatives. For more detailed information see Product Specificity Chart below.
Support: 4% cross-linked agarose beads
Capacity: > 4 µg RFP per µl of packed beads (= 2µl of slurry)
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
RFP Selector Kit: Our RFP Selector Kit (Cat. No. N0410-Kit) contains 500 µl RFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 20 MiniSpin columns.
|Product name||Resin||Amount||Net price (€)||Catalog Number||Order at|
|RFP Selector||4% Agarose CL||2000 µl slurry for up to 100 reactions||see webshop||N0410|
|Selector Control||4% Agarose CL||400 µl slurry for up to 20 reactions||see webshop||N0010-S|
|MiniSpin Columns||n.a.||10 columns||see webshop||A1001-S|
|MiniSpin Columns||n.a.||50 columns||see webshop||A1001-L|
This information is provided without liability and may be subject to change without prior notification.