For microscopy applications, NanoTag Biotechnologies offers a range of single-domain antibody-based FluoTag products displaying significant advantages over conventional antibodies.
Our FluoTags can freely move in crowded environments like fixed tissue or cells. Their fast diffusion allows them to find epitopes faster. They also can reach epitopes that conventional IgGs have no access to.
Steric hindrance is minimal with our FluoTags. This means that FluoTags will find more epitopes than conventional IgGs. This results in a more uniform decoration of the structures under investigation.
Staining of live or poorly fixed samples is often prone to clustering artifacts. FluoTags are monovalent and recognize only one epitope. They therefore efficiently prevent clustering of target molecules or organelles.
Our FluoTag-X series features multiple fluorophores per affinity probe. For maximal brightness, FluoTag-X4 products even contain a blend of probes recognizing different epitopes on the same target.
We can couple FluoTags to your favorite fluorophore. No need to match the secondary antibody to the species of your primary. In addition, direct coupling can significantly shorten your experimental time.
FluoTags contain a fixed number of fluorophores at defined positions. In our FluoTag-Q series, for example, each fluorophor is linked to exactly one FluoTag molecule at a position less than 1.5 nm apart from the epitope.
FluoTags locate the fluorophores 10-15 nm closer to their targets than classical detection systems. This improves the localization accuracy required for all modern super-resolution microscopy techniques.
Our FluoTag-Q series feature high affinity monovalent binding, low steric constraints and a defined fluorophore number per molecule. This ensures a linear relation between target molecule number and fluorescence intensity.
Count on our FluoTag-Q series! One target molecule per FluoTag – One FluoTag per fluorophore. Together with the high affinity of our reagents, these features enable you to even count your target molecule of interest.
Our current product lines are based on recombinant single-domain antibodies (sdAbs) derived from llamas or alpacas. This type of affinity Tags (also known as “Nanobodies”; trademark owned by Ablynx) provides significant advantages over conventional IgG molecules. All of our Tags are produced in-house. They are highly specific with affinities tailored for a wide range of applications.