A, Pull-down of GFP from a mixture of GFP, mCherry and mTagBFP
B, Immunoprecipitation of GFP (•) from HeLa lysate.
In/Ft: 1/1000 of input and non-bound material.
E: Eluate from 1 µl of beads
* Specific maturation band from GFP family members
C, Control experiment using functionalized beads lacking sdAbs.
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below. GFP Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see Product Specificity Chart below.
Capacity: > 4 µg GFP per µl of packed beads (i.e. 2 µl of slurry)
Support: 4% cross-linked agarose beads
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
GFP Selector Kit: Our GFP Selector Kit (Cat. No. N0310-Kit) contains 500 µl GFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 20 MiniSpin columns.
|Product name||Resin||Amount||Net price (€)||Catalog Number||Order at|
|GFP Selector||4% Agarose CL||2000 µl slurry for up to 100 reactions||see webshop||N0310|
|Selector Control||4% Agarose CL||400 µl slurry for up to 20 reactions||see webshop||N0010-S|
|MiniSpin Columns||na||10 columns||see webshop||A1001-S|
|MiniSpin Columns||na||50 columns||see webshop||A1001-L|
This information is provided without liability and may be subject to change without prior notification.