FluoTag®-X4 anti-RFP

FluoTag®-X4 anti-RFP

STED image of HeLa cells expressing the mitochondrial marker Tom70-mCherry, stained with FluoTag-X4 anti-RFP AbberiorStar635P (red).

FluoTag®-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.

Specificity: Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see Product Reactivity Chart below.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:250
Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.

Product List

Product name Coupled to Volume Net price (€) Catalog Number Order at Customized
FluoTag-X4 anti-RFP see above 20 µl see webshop N0404

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FluoTag-X4 anti-RFP see above 200 µl see webshop N0404

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This information is provided without liability and may be subject to change without prior notification.

References

West et al. (2018) The pro-apoptotic JNK scaffold POSH/SH3RF1 mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia, Human Molecular Genetics, Volume 27, Issue 8, 15 April 2018, Pages
Human Molecular Genetics, Volume 27, Issue 8, 15 April 2018, Pages 1382–1395