STED image of HeLa cells expressing the mitochondrial marker Tom70-mCherry, stained with FluoTag-X4 anti-RFP AbberiorStar635P (red).
FluoTag®-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.
Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see Product Reactivity Chart below.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:250
Available conjugates: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Alexa Fluor® 647, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5, DBCO and biotin. For other fluorophores or conjugates please contact us.
|Product name||Coupled to||Volume||Net price (€)||Catalog Number||Order at||Customized|
|FluoTag-X4 anti-RFP||see above||20 µl||see webshop||N0404||Contact Us|
|FluoTag-X4 anti-RFP||see above||200 µl||see webshop||N0404||Contact Us|
This information is provided without liability and may be subject to change without prior notification.
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Neuron 103, 1–14 July 3
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