FluoTag®-X4 anti-Rabbit IgG

FluoTag®-X4 anti-Rabbit IgG

This FluoTag®-X4 anti-Rabbit (N2404) is a species-specific FluoTag® directed against immunoglobulins from Rabbit. It has a higher affinity to Rabbit IgG molecules than our previous FluoTag-X2 anti-Rabbit IgG (Cat. no. N1002). We were able to identify two fantastic single-domain antibodies (sdAbs) that can be blended and both bind simultaneously to a Rabbit IgG. This allowed us to introduce the Fluotag®-X4 version. In our FluoTag-X4 series, two fluorophores are site-specifically coupled to each individual FluoTag sdAb clone. Since IgGs are homodimer macromolecules, each Rabbit IgG is decorated by 4 sdAbs when incubated with our FluoTag®-X4 anti-Rabbit, resulting in up to eight fluorophores per single IgG molecule. This ensures extra-bright signals and due to the monovalent nature of FluoTags, there are no probe-induced artifacts by primary and secondary antibody clusters of target molecules, leading to better epitope accessibility and more precise localization for super-resolution microscopy. Since our FluoTags are recombinantly expressed, this blend has no batch-to-batch variation.

Specificity: Recognizes the native Fc from Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Produced in: E. coli
Concentration: 2.5 µM of each sdAb, 10 µM fluorophore
Recommended dilution: 1:250
Available conjugates: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Alexa Fluor® 647, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5, DBCO and biotin. For other fluorophores or conjugates please contact us.

Relevant Documents

Product List

Product name Coupled to Volume Net price (€) Catalog Number Order at Customized
FluoTag-X4 anti-Rabbit IgG see above 500 µL see webshop N2404

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FluoTag-X4 anti-Rabbit IgG see above 2 mL (4 x 500 µL) see webshop N2404

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This information is provided without liability and may be subject to change without prior notification.