HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 were PFA-fixed and stained with FluoTag-X4 anti-GFP AbberiorStar635P. Shown is an overlay of the FluoTag-X4 anti-GFP signal (red) with DAPI
FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.
Specificity: Recognizes native GFP, mEGFP, superfolder GFP, Emerald and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see the Product Reactivity Chart below.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:500
Available conjugates: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Alexa Fluor® 647, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5, DBCO and biotin. For other fluorophores or conjugates please contact us.
|Product name||Coupled to||Volume||Net price (€)||Catalog Number||Order at||Customized|
|FluoTag-X4 anti GFP||see above||20 µl||see webshop||N0304||Contact Us|
|FluoTag-X4 anti GFP||see above||200 µl||see webshop||N0304||Contact Us|
This information is provided without liability and may be subject to change without prior notification.
Hussain et al. (2018) Inhibition of oxidative stress in cholinergic projection neurons fully rescues aging-associated olfactory circuit degeneration in Drosophila. Elife. 2018 Jan 18;7
PMID: 29345616 DOI: 10.7554/eLife.32018
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