FluoTag®-X4 anti-GFP

FluoTag®-X4 anti-GFP

HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 were PFA-fixed and stained with FluoTag-X4 anti-GFP AbberiorStar635P. Shown is an overlay of the FluoTag-X4 anti-GFP signal (red) with DAPI

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.

Antigen: GFP
Specificity: Recognizes native GFP, mEGFP, superfolder GFP, Emerald and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see the Product Reactivity Chart below.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:250
Available fluorohores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.

Product List

Product name Coupled to Volume Net price (€) Catalog Number Order at Customized
FluoTag-X4 anti GFP see above 20 µl see webshop N0304

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FluoTag-X4 anti GFP see above 200 µl see webshop N0304

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This information is provided without liability and may be subject to change without prior notification.

References

Hussain et al. (2018) Inhibition of oxidative stress in cholinergic projection neurons fully rescues aging-associated olfactory circuit degeneration in Drosophila. Elife. 2018 Jan 18;7
PMID: 29345616 DOI: 10.7554/eLife.32018

Fulterer et al. (2018) Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses
DOI:https://doi.org/10.1016/j.celrep.2018.03.126