PFA fixed COS cells transfected with mScarlet-i-tubullin (A) stained with FluoTag-X2 anti-mScarlet-i Atto488 (B). Overlay in (C)
FluoTag®-X2 anti-mScarlet-i is derived from an in-house developed single-domain antibody (sdAb) recognizing mScarlet-i with high affinity and specificity. Our FluoTag-X2 series features two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X2 series can thus improve the localization accuracy by 10-15 nm. Both features – enhanced brightness and precise fluorophore placement – render our FluoTag-X2 products superior tools for all microscopy techniques. Specificity: Recognizes mScarlet-i and some mRFP-derived red fluorescent proteins like and mCherry in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see the Product Reactivity Chart. Concentration: 5 µM protein, 5 µM fluorophore Recommended dilution for IF/ ICC: 1:500
Recognizes mScarlet-i in its native conformation. Cross-reacts with some mRFP-derived red fluorescent proteins like mCherry.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:500
Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.
|Product name||Coupled to||Volume||Net price (€)||Catalog Number||Order at||Customized|
|FluoTag-X2 anti-mScarlet-i||see above||20 µl||see webshop||N1302||Contact Us|
|FluoTag-X2 anti-mScarlet-i||see above||200 µl||see webshop||N1302||Contact Us|
This information is provided without liability and may be subject to change without prior notification.
Mahen (2018) Stable centrosomal roots disentangle to allow interphase centriole independence
PLoS Biol 16(4): e2003998. https://doi.org/10.1371/journal.pbio.2003998