COS-7 cell transfected with Tom70-ALFA (mitochondria) and stained with FluoTag-X2 anti-ALFA AlexaFluor647. False colour representation in green.
FluoTag®-X2 anti-ALFA is derived from an in-house developed single-domain antibody (sdAb) that recognize a small rationally-designed epitope tag: the ALFA-tag. Our FluoTag-X2 series binds in a monovalent fashion with high affinity and specificity irrespective if the sample is alive or fixed with 4% paraformaldehyde, 2% glutaraldehyde or methanol. Additionally, like all X2 FluoTags it features two site-specifically coupled fluorophores per sdAb. Owing to the small size of our FluoTags and the short ALFA-tag (15 aminoacids), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-Termini or in between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Selector resins with physiological elution capability. Therefore, this ALFA-tag and the companion FluoTags-X2 anti ALFA and ALFA-Selectors are the perfect choice for fluorescent-based experiments that want to be combined with biochemical purifications for mass-spectrometry or any other high-end application.
Recognizes the ALFA-tag
Produced in: E. coli
Concentration: 2.5 µM protein and 5 µM fluorophore
Recommended dilution: 1:500
Available Fluorophores: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.
|Product name||Coupled to||Volume||Net price (€)||Catalog Number||Order at||Customized|
|FluoTag-X2 anti-ALFA||see above||20 µL||see webshop||N1502||Contact Us|
|FluoTag-X2 anti-ALFA||see above||200 µL||see webshop||N1502||Contact Us|
This information is provided without liability and may be subject to change without prior notification.