Epifluorescence image of HeLa cells expressing the mitochondrial marker Tom70-mCherry. Cells were immunostained with FluoTag-Q anti-RFP Atto647N (red). Nuclei were stained with DAPI (blue).
FluoTag®-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. For a complete specificity chart see the Product Reactivity Chart below. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see the Product Reactivity Chart below.
Concentration: 5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:1000
Available conjugates: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Alexa Fluor® 647, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5, DBCO and biotin. For other fluorophores or conjugates please contact us.
|Product name||Coupled to||Volume||Net price (€)||Catalog Number||Order at||Customized|
|FluoTag-Q anti-RFP||see above||20 µl||see webshop||N0401||Contact Us|
|FluoTag-Q anti-RFP||see above||200 µl||see webshop||N0401||Contact Us|
This information is provided without liability and may be subject to change without prior notification.
Gombkoto et al. (2019) Contribution of the Basal Forebrain to Corticocortical Network Interactions