FluoTag®-Q anti-GFP

FluoTag®-Q anti-GFP

Confocal image of HeLa cells expressing the mitochondrial marker Tom70-GFP. Cells were immunostained with FluoTag-Q anti-GFP Atto647N (green).

FluoTag®-Q anti-GFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes GFP and its most common derivatives with high affinity and specificity. For a complete specificity chart see the Product Reactivity Chart below. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Antigen: GFP
Specificity: Recognizes native GFP, mEGFP, superfolder GFP, Emerald and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see the Product Reactivity Chart below.
Produced in: E. coli
Concentration: 5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:1000
Available conjugates: Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Alexa Fluor® 647, Atto 488, Atto 542, Atto 565, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5, DBCO and biotin. For other fluorophores or conjugates please contact us.
: Datasheet

Product List

Product name Coupled to Volume Net price (€) Catalog Number Order at Customized
FluoTag-Q anti-GFP see above 20 µl see webshop N0301

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FluoTag-Q anti-GFP see above 200 µl see webshop N0301

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This information is provided without liability and may be subject to change without prior notification.

References

Wagner et al. (2019) Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression DOI:https://doi.org/10.1016/j.celrep.2019.06.005

Seitz et al. (2019) GFP nanobodies reveal recently-exocytosed pHluorin molecules Scientific Reports volume 9, Article number: 7773 (2019)

Thevathasan et al. (2019) Nuclear pores as versatile reference standards for quantitative superresolution microscopy
https://doi.org/10.1038/s41592-019-0574-9