The recombinant sdAb anti-SARS-CoV-2 Spike protein S1 Rabbit Fc-fusion is a genetic fusion of the single-domain antibody anti-SARS-CoV-2 Spike protein S1 to a Rabbit Fc domain. This bivalent tool binds the SARS-CoV-2 RBD domain of the S1 protein with high affinity and is ideally suited for ELISA and IHC staining. The fused Rabbit Fc allows for conventional secondary detections tools used in automated high-thoughput-screening devices. The minibody preserves the specificity and high affinity binding of the anti-S1 SARS-CoV-2 single-domain antibody and can neutralize infections as it efficiently competes with ACE-2 receptor binding as shown by the team of Alejandro Rojas-Fernandez at the Institute of Medicine from the Universidad Austral de Chile.
RBD of S1 protein from SARS-CoV-2
Produced in: HEK cells
Formulation: 100 µg lyophilzed from PBS
Recommended dilution for IF/IHC-P/ELISA: 1:500/ 10 µg/mL / 1:1000
Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity Nanobody doi: https://doi.org/10.1101/2020.06.09.137935