ALFA Selector PE (for "peptide elutable") is an innovative affinity resin designed for the highly efficient purification of native ALFA-tagged target proteins under physiological conditions. For general information about the ALFA system click here. ALFA Selector PE is based on a high-affinity (Kd = 11 nM) single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. ALFA Selector PE is optimized for an efficient competitive elution under physiological conditions using ALFA elution peptide. For this purpose, every unit of ALFA Selector PE is supplied with 1 mg of ALFA elution peptide, which is sufficient for 2.5 mL of elution buffer. Alternatively, elution can be performed under acidic or denaturing conditions. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution, even under harsh conditions (e.g. SDS sample buffer). ALFA Selector PE thus features high affinity and superior capacity for ALFA fusion proteins while showing negligible non-specific background. ALFA Selector PE is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). ALFA Selector PE thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Datasheet: Download PDF
Recognizes ALFA-tagged proteins
Capacity: > 4 µg ALFA-tagged GFP per µl of packed beads (i.e. 2 µl of slurry)
Support: 4% cross-linked agarose beads
Formulation: 50% slurry in 20% ethanol / PBS; lyophylized peptide
Recommended for: Immunoprecipitation, Mass Spectrometry
ALFA elution peptide (for elution): 1 mg lyophilized peptide (sufficient for 2.5 ml)
|Product name||Resin||Amount||Net price (€)||Catalog Number||Order at|
|ALFA Selector PE||4% Agarose CL||2 mL slurry for up to 100 reactions, incl. 1 mg ALFA elution peptide||see webshop||N1510|
|ALFA elution peptide||10 mg||see webshop||N1520-L|
|Selector Control||4% Agarose CL||400 µl slurry for up to 20 reactions||see webshop||N0010-S|
|MiniSpin Columns||10 columns||see webshop||A1001-S|
|MiniSpin Columns||50 columns||see webshop||A1001-L|
This information is provided without liability and may be subject to change without prior notification.