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N2741_sdAb anti-Mouse IgG2 Halo fusion

Immunocytochemistry of PFA-fixed COS-7 cells stained with mouse anti-Lamin AC primary antibody (isotype IgG2) and sdAb anti-Mouse IgG2 Halo fusion (Cat. No. N2741; 10nM) and detected with fluorophore-labeled Halo ligand.

sdAb anti-Mouse IgG2a/b Halo fusion

Cat No: N2741 Category:

275,00 €

The sdAb anti-Mouse IgG2 Halo fusion combines a species-specific single-domain antibody (sdAb) targeting the mouse IgG2 (Smart Secondaries®) with a dehalogenase-based technology. The Halo protein tag is fused to the C-terminus of the sdAb anti-Mouse IgG2.

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The sdAb anti-Mouse IgG2 Halo fusion combines a species-specific single-domain antibody (sdAb) targeting the mouse IgG2 with a dehalogenase-based technology. The Halo protein tag sequence is fused to the C-terminus of the sdAb anti-Mouse IgG2.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications. When combined with a fluorescent Halo ligand, the sdAb anti-Mouse IgG2 Halo fusion can be used in immunofluorescence as a secondary reagent specifically binding to the mouse IgG2 (IgG2a and IgG2b) primary antibodies or our recombinant heavy-chain antibodies (rHcAb mouse IgG2 Fc fusion).

To learn more about the Smart Secondaries®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
- 50 µg N2741 -
Related Products: -
Clone: 14A4
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: For IF (ICC), use the sdAb anti-Mouse IgG1 Halo fusion at 1:500 dilution.
Capacity: N/A
Antigen: -
Targets: Mouse IgG2
Specificity: Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

sdAb anti-Mouse IgG2 Halo fusion is lyophilized from PBS pH 7.4 with 2% BSA (US-Origin). After reconstitution in 200 μL of sterile 50% glycerol (v/v) in deionized water, the final concentration of sdAb anti-Mouse IgG2 Halo fusion is 0.25 mg/mL. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 47,8
Ext Coef: 78630
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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