sdAb anti-Mouse IgG1 Halo fusion

Cat No: N2041 Category:

275,00 

The sdAb anti-Mouse IgG1 Halo fusion combines a species-specific single-domain antibody (sdAb) targeting the mouse IgG1 (Smart Secondaries®) with a dehalogenase-based technology. The sdAb anti-Mouse IgG1 is fused to the Halo protein tag sequence at its C-terminus.

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The sdAb anti-Mouse IgG1 Halo fusion combines a species-specific single-domain antibody (sdAb) targeting the mouse IgG1 with a dehalogenase-based technology. The sdAb anti-Mouse IgG1 is fused to the Halo protein tag sequence at its C-terminus.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications. When combined with a fluorescent Halo ligand, the sdAb anti-Mouse IgG1 Halo fusion can be used in immunofluorescence as a secondary reagent specifically binding the conventional mouse IgG1 primary antibodies or our recombinant heavy-chain antibodies (rHcAb mouse IgG1 Fc fusion).

To learn more about the Smart Secondaries®, please visit our Technology section here.

 

Variations:
Conjugation Amount Cat No. RRID
- 50 µg N2041 -
Related Products: -
Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: For IF (ICC), use the sdAb anti-Mouse IgG1 Halo fusion at 1:500 dilution.
Capacity: N/A
Antigen: Mouse IgG1
Targets: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

sdAb anti-Mouse IgG1 Halo fusion is lyophilized from PBS pH 7.4 with 2% BSA (US-Origin). After reconstitution in 200 μL of sterile 50% glycerol (v/v) in deionized water, the final concentration of sdAb anti-Mouse IgG1 Halo fusion is 0.25 mg/mL. For more details, click the Protocols button above and check Reconstitution and Storage.

kDa: 48,4
Ext Coef: 82640
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.

Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.