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N1241_sdAb-anti-Mouse-KLC-Halo-fusion_NanoTag

Immunocytochemistry of PFA-fixed HeLa cells. A False color representation of HeLa cells stained with mouse anti-alpha Tubulin primary antibody (Synaptic Systems Cat. No. 302 211; 1:1000 dilution) and sdAb anti-Mouse Ig kappa light chain Halo fusion (Cat. No. N1241; 10nM) and detected with fluorophore-labeled Halo ligand (magenta). B False color representation of corresponding DAPI staining (blue). C Merge of A and B.

sdAb anti-Mouse Ig kappa light chain Halo fusion

Cat No: N1241 Category:

275,00 €

The sdAb anti-Mouse kappa light chain Halo fusion combines a species-specific single-domain antibody (sdAb) binding to all mouse Ig isotypes carrying a kappa light chain (Smart Secondaries®) with a dehalogenase-based technology. The Halo protein tag is fused to the C-terminus of the sdAb anti-Mouse kappa light chain.

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Most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda, or kappa light chain (KLC).

The sdAb anti-Mouse kappa light chain Halo fusion combines a species-specific single-domain antibody (sdAb) binding to all mouse Ig isotypes carrying a kappa light chain with a dehalogenase-based technology. The Halo protein tag sequence is fused to the C-terminus of the sdAb anti-Mouse kappa light chain.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications. When combined with a fluorescent Halo ligand, the sdAb anti-Mouse kappa light chain Halo fusion can be used in immunofluorescence as a secondary reagent specifically binding to all mouse Ig isotypes carrying a kappa light chain.

To learn more about the Smart Secondaries®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
- 50 µg N1241 -
Related Products:

sdAbs anti-Mouse Ig kappa light chain (Cat. No. N1205)

FluoTag®-X2 anti-Mouse Ig kappa light chain (Cat. No. N1202)

sdAb anti-Mouse IgG1 Halo fusion (Cat. No. N2041)

sdAb anti-Mouse IgG2 Halo fusion (Cat. No. N2741)
Clone: 1A23
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: For IF (ICC), use the sdAb anti-Mouse KLC Halo fusion at 1:500 dilution.
Capacity: N/A
Antigen: -
Targets: Mouse kappa light chain
Specificity: Specifically recognizes Mouse Immunoglobulin kappa light chains. Does not cross-react with antibodies from species like: Rat, Guinea Pig, Rabbit, Chicken or Goat.
Formulation:

sdAb anti-Mouse Ig kappa light chain Halo fusion is lyophilized from PBS pH 7.4 with 2% BSA (US-Origin). After reconstitution in 200 μL of sterile 50% glycerol (v/v) in deionized water, the final concentration of sdAb anti-Mouse Ig kappa light chain Halo fusion is 0.25 mg/mL. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 48,6
Ext Coef: 91580
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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