mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor, have a look at our specificity chart in the resource section.
| Variations: |
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| Related Products: | - | ||||||||||||||||
| Clone: | 1E2 | ||||||||||||||||
| Host: | Alpaca | ||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||
| Application: | IF, IHC | ||||||||||||||||
| Dilution: | N/A | ||||||||||||||||
| Capacity: | N/A | ||||||||||||||||
| Antigen: | - | ||||||||||||||||
| Targets: | mNeonGreen | ||||||||||||||||
| Specificity: | Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives. | ||||||||||||||||
| Formulation: | DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. | ||||||||||||||||
| kDa: | 16.8 | ||||||||||||||||
| Ext Coef: | 35598 | ||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||
| Storing: | Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | ||||||||||||||||
| Protocols: |
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. |
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| References: | - | ||||||||||||||||
| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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