sdAb anti-mNeonGreen

Cat No: N3205 Category:

275,00 625,00 

A single-domain antibody (sdAb) that recognizes mNeonGreen (mNG), which is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume.

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mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.

An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”

Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor. Check out our Specificity Chart for more details.

Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N3205-250ug AB_3076073
Biotin 250 μg N3205-Biotin AB_3076074
DBCO 250 μg N3205-DBCO AB_3076075
Related Products:

FluoTag®-X2 anti-mNeonGreen (Cat. No. N3202)

mNeonGreen Selector (Cat. No. N3210)

Clone: 1E2
Host: Alpaca
Produced in: E.coli
Application:

IF (ICC)

IHC

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.

Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mNeonGreen
Specificity: Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.

kDa: 16.8
Ext Coef: 35598
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.

Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and Specificity Chart.

References:
  1. Deutz LN, Sarıkaya S, Dickinson DJ. Membrane extraction in native lipid nanodiscs reveals dynamic regulation of Cdc42 complexes during cell polarization. Biophys J. Published online November 23, 2023. doi:10.1016/j.bpj.2023.11.021 (N3205-Biotin; Table S1; Microfluidic devices/coverslips coating for TIRF microscopy)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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