sdAb anti-mNeonGreen

Cat No: N3205 Category:

250,00 600,00 

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mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.

Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor, have a look at our specificity chart in the resource section.

MSDS
Variations:
Conjugation Amount Cat No.
unconjugated C-terminal Cys 250 μg N3205-250µg
Biotin 250 μg N3205-Biotin
DBCO 250 μg N3205-DBCO
Related Products: -
Clone: 1E2
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: N/A
Capacity: N/A
Antigen: mNeonGreen
Specificity: Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives.
Formulation: DBCO and biotin conjugated sdAbs are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.
kDa: 16.8
Ext Coef: 35598
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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