mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor, have a look at our specificity chart in the resource section.
Variations: |
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Related Products: | - | ||||||||||||
Clone: | 1E2 | ||||||||||||
Host: | Alpaca | ||||||||||||
Produced in: | E.coli | ||||||||||||
Application: | IF, IHC | ||||||||||||
Dilution: | N/A | ||||||||||||
Capacity: | N/A | ||||||||||||
Antigen: | - | ||||||||||||
Targets: | mNeonGreen | ||||||||||||
Specificity: | Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives. | ||||||||||||
Formulation: | DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. | ||||||||||||
kDa: | 16.8 | ||||||||||||
Ext Coef: | 35598 | ||||||||||||
Shipping: | Ambient temperature | ||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | ||||||||||||
Protocols: |
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. |
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References: | - | ||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |