275,00 € – 625,00 €
A single-domain antibody (sdAb) that recognizes mNeonGreen (mNG), which is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume.
mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor. Check out our Specificity Chart for more details.
Variations: |
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Clone: | 1E2 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: |
IF (ICC) IHC Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | - | ||||||||||||||||
Targets: | mNeonGreen | ||||||||||||||||
Specificity: | Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives. | ||||||||||||||||
Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | 16.8 | ||||||||||||||||
Ext Coef: | 35598 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and Specificity Chart. |
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References: |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |