275,00 € – 625,00 €
sdAb anti-Human IgG (clone 2E5) is an effective sdAb-based secondary reagent from our Smart Secondaries® product line. It specifically targets Human IgG immunoglobulins, providing exceptional sensitivity and reliability for demanding immunolabeling and imaging applications.
sdAb anti-Human IgG (clone 2E5) is a single-domain antibody (sdAb, nanobody, VHH) with high specificity for the constant Fc region of Human IgG isotypes. The reagent recognizes human IgG1 and IgG4 with high affinity. Weaker binding is observed to Human IgG2 (see Figure). It is part of our Smart Secondaries® product line of sdAb-based secondary reagents. sdAb anti-Human IgG (clone 2E5) is an ideal secondary tool for high-precision applications involving primary Human antibodies of the IgG1 or IgG4 isotype.
We provide this product conjugated to various labels and offer custom conjugation services with most commercially available labels, provided these are available in a thiol-reactive activated form.
Variations: |
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Clone: | 2E5 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: | ELISA, IF, IHC, WB | ||||||||||||||||
Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | Human IgG | ||||||||||||||||
Targets: | Human IgG | ||||||||||||||||
Specificity: | Recognizes the Fc domain of native Human IgGs. Human IgG1 +++, Human IgG2 +, Human IgG4 +++, no significant binding to Human IgG3. No significant cross-reaction with Mouse, Rabbit, GuineaPig, Chicken, Rat, Goat IgGs or Human IgA, IgD, IgE or IgM. | ||||||||||||||||
Formulation: |
Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. The HRP-conjugated sdAbs are lyophilized from HRP protector buffer and should be reconstituted with 200 µL pure deionized water for storage at 4°C or with 200 µL high purity, sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | 15.4 | ||||||||||||||||
Ext Coef: | 28670 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks (Unconjugated and Biotin-conjugated sdAbs) or at 4°C for up to 6 months (HRP-conjugated sdAbs). For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page. |
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References: | - | ||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |