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FluoTag-X2 anti-CNPase AF568

PFA-fixed hippocampal primary neuronal cultures from rats were stained using a fluorescently labeled version of sdAb anti-CNPase, the FluoTag®-X2 anti-CNPase conjugated to AZDye 568 (Cat. No. N4102-AF568, dilution 1:500). A Greyscale image of the staining performed with N4102-AF568. B False color representation of A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

sdAb anti-CNPase

Cat No: N4105 Category:

Price range: 290,00 € through 655,00 €

Single-domain antibody that recognizes mouse CNPase with high affinity and specificity. It can be used as a marker for myelinating cells.

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sdAb anti-CNPase is an in-house developed single-domain antibody (sdAb) that specifically recognizes a CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase), also known as CNP1. CNPase is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system. It may also be involved in the RNA metabolism of  myelinating cells of the central nervous system (CNS), the oligodendrocytes. CNPase is frequently used as a marker for oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS).

We provide this product conjugated to various labels and offer custom conjugation services with most commercially available labels, provided these are available in a thiol-reactive activated form.

MSDS
sdAb unconjugated
sdAb Biotin/DBCO
Protocols
Reconstitution and Storage
Maleimide labeling
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N4105-250ug AB_3675683
Biotin 250 μg N4105-Biotin AB_3675684
Related Products:

FluoTag®-X2 anti-CNPase (Cat. No. N4102)

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Clone: 1D5
Host: Alpaca
Produced in: E.coli
Application:

IF (ICC), IHC, ELISA (not specifically tested yet)
Dilution: Not specifically tested.
Capacity: N/A
Antigen: CNPase
Targets: CNPase
Specificity: Recognizes mouse CNPase.
Formulation:

Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C.

For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 16.4
Ext Coef: 28608
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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