N4205-250ug conjugated with FITC-maleimide used for co-staining of human PMBCs (right panel) with anti-CD2 and anti-CD19 (APC) in FACS. Control of human PBMCs stained with conventional CD2, CD19 (APC) and CD45 antibodies (FITC) (left panel). Courtesty of Niklas Engels UMG Göttingen.
STED image of RAMOS B cells stained with FluoTag-X2 anti-CD45 AbberiorSTAR635P. Courtesy of Felipe Opazo UMG Göttingen.
CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leukocytes, and which plays a crucial role in the function of these cells.
| Variations: |
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| Related Products: | - | ||||||||||||||||
| Clone: | 4B7 | ||||||||||||||||
| Host: | Alpaca | ||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||
| Application: | FACS | ||||||||||||||||
| Dilution: | N/A | ||||||||||||||||
| Capacity: | N/A | ||||||||||||||||
| Antigen: | - | ||||||||||||||||
| Targets: | CD45 | ||||||||||||||||
| Specificity: | - | ||||||||||||||||
| Formulation: | DBCO and biotin conjugated sdAbs are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0, 150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water (unconjugated sdAbs), or with 250 µL 50% glycerol for storage at -80 °C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. | ||||||||||||||||
| kDa: | 16.4 | ||||||||||||||||
| Ext Coef: | 24138 | ||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||
| Storing: | Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C. | ||||||||||||||||
| Protocols: |
Look at detailed protocols and our specificity chart in our Resource Section. |
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| References: | - | ||||||||||||||||
| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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