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N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_costaining PSD95_100x_NanoTag

Indirect immunostaining of PFA-fixed rat hippocampal primary neuronal cultures. A Staining of neurons with Recombinant anti-Synaptotagmin 1 antibody (7A51) Mouse IgG1 Fc-fusion (Cat. No. N4382, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Mouse IgG1 Alexa647 (Cat. No. N2002-AF647, 1:1000; magenta). B Co-staining with FluoTag®-X2 anti-PSD95 Atto488 (Cat. No. N3702-At488, 1:1000; green). C Merge of A and B with DAPI signal for nuclei (blue).

 N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_costaining PSD95_NanoTag

Indirect immunostaining of PFA-fixed rat hippocampal primary neuronal cultures. A Staining of neurons with Recombinant anti-Synaptotagmin 1 antibody (7A51) Mouse IgG1 Fc-fusion (Cat. No. N4382, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Mouse IgG1 Alexa647 (Cat. No. N2002-AF647, 1:1000; magenta). B Co-staining with FluoTag®-X2 anti-PSD95 Atto488 (Cat. No. N3702-At488, 1:1000; green). C Merge of A and B with DAPI signal for nuclei (blue).

 N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_NanoTag

Indirect immunostaining of PFA-fixed sagittal rat hippocampus section using Recombinant anti-Synaptotagmin 1 (A51) antibody Mouse IgG1 Fc-fusion (Cat. No. N4382, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Mouse IgG1 AZDye568 (Cat. No. N2002-AF568, 1:500; red). Cell nuclei are counterstained with DAPI (blue). Scale bar: 50 µm.

 N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_costaining PSD95_100x_NanoTag

Indirect immunostaining of PFA-fixed rat hippocampal primary neuronal cultures. A Staining of neurons with Recombinant anti-Synaptotagmin 1 antibody (A51) Rabbit IgG Fc-fusion (Cat. No. N4383, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Rabbit IgG Alexa647 (Cat. No. N2402-AF647, 1:1000; magenta). B Co-staining with FluoTag®-X2 anti-PSD95 Atto488 (Cat. No. N3702-At488; 1:1000; green). C Merge of A and B with DAPI signal for nuclei (blue).

 N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_costaining PSD95_NanoTag

Indirect immunostaining of PFA-fixed rat hippocampal primary neuronal cultures. A Staining of neurons with Recombinant anti-Synaptotagmin 1 antibody (A51) Rabbit IgG Fc-fusion (Cat. No. N4383, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Rabbit IgG Alexa647 (Cat. No. N2402-AF647, 1:1000; magenta). B Co-staining with FluoTag®-X2 anti-PSD95 Atto488 (Cat. No. N3702-At488; 1:1000; green). C Merge of A and B with DAPI signal for nuclei (blue).

 N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_NanoTag

Indirect immunostaining of PFA-fixed sagittal rat hippocampus section using Recombinant anti-Synaptotagmin 1 (A51) antibody Rabbit IgG Fc-fusion (Cat. No. N4383, 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Rabbit IgG AZDye568 (Cat. No. N2402-AF568, 1:500; red). Cell nuclei are counterstained with DAPI (blue). Scale bar: 50 µm.
N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_costaining PSD95_100x_NanoTag
N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_costaining PSD95_NanoTag
N4382_Recombinant anti-SYT1 antibody (A51)_Mouse IgG1 Fc-fusion_NanoTag
N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_costaining PSD95_100x_NanoTag
N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_costaining PSD95_NanoTag
N4383_Recombinant anti-SYT1 antibody (A51)_Rabbit IgG Fc-fusion_NanoTag

Recombinant anti-Synaptotagmin 1 antibody (A51) rHcAb

Cat No: N/A Category:

420,00 €

The recombinant heavy-chain anti-Synaptotagmin 1 antibody (A51) is a genetic fusion of our camelid anti-Synaptotagmin 1 single-domain antibody (sdAb) to either a Mouse IgG1 or a Rabbit IgG Fc domain. It specifically recognizes rat, mouse and human SYT1.

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The recombinant heavy-chain anti-Synaptotagmin 1 antibody (A51) is a genetic fusion of our camelid anti-Synaptotagmin 1 single-domain antibody (sdAb) to either a Mouse IgG1 or a Rabbit IgG Fc domain. The resulting bivalent binders combine the high target specificity of sdAbs with maximal flexibility and coherence needed for Fc-domain-based applications. The antibody has the specificity of the nanobody clone #A51 (fully characterized here) and recognizes the cytoplasmic domain of rat, mouse, and theoretically also human Synaptotagmin 1. It can be detected using conventional secondary reagents or with our secondary nanobodies (Smart Secondaries®), which offer advantages in applications where secondary antibody clustering is a concern or where a smaller probe size is required. More information on recombinant heavy-chain antibodies (rHcAb) is available here.

Synaptotagmin 1 (SYT1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two calcium-binding domains in their intracellular portion. Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.

Note: We offer two anti-Synaptotagmin 1 sdAb clones, #A51 and #7D5, with comparably high specificity and performance. Clone selection can be based on availability or preference for published references. Full characterization data have been published for nanobody clone #A51 here.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Mouse IgG1 Fc-fusion 100 µg N4382 AB_3741790
Rabbit IgG Fc-fusion 100 µg N4383 AB_3741791
Related Products:

FluoTag®-X2 anti-Synaptotagmin (A51) (Cat. No. N4302)

sdAb anti-Synaptotagmin (A51)(Cat. No. N4305)

View related products
Clone: A51
Host: Alpaca
Produced in: HEK
Application: IF, IHC
Dilution: 1:500 (IF), 1:1000 or 1 µg/mL (IHC)
Capacity: N/A
Antigen: Synaptotagmin 1 (cytosolic)
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic C2A domain of rat, mouse and by homology also human Synaptotagmin 1 (other species not tested).
Formulation:

Lyophilized from PBS pH 7.4. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.

Limited Use Label License: Internal Research Use Only
The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.