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Indirect immunostaining of PFA fixed COS cells expressing POM121-GFP with recombinant anti-GFP antibody (Cat. No. N0383; 1:500) and decorated with FluoTag®-X4 anti-Rabbit AZDye 568 (Cat. No. N2404-AF568-S; dilution 1:500), the merge of both channels is represented in orange. Nuclei were visualized by DAPI staining (blue).

Recombinant anti-GFP antibody (rHcAb)

Cat No: N/A Category:

400,00 €

The recombinant heavy-chain anti-GFP antibody is a genetic fusion of our established camelid anti-GFP single-domain antibody (sdAb) to a Rabbit IgG Fc domain.

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The recombinant heavy-chain anti-GFP antibody is a genetic fusion of our established camelid anti-GFP single-domain antibody (sdAb) to a Rabbit IgG Fc domain. This antibody preserves the specificity and high-affinity binding of the anti-GFP sdAb and adds the features of a conventional anti-rabbit antibody. It can be used in many applications based on immunodetection (IF, IHC, IP). However, this recombinant anti-GFP antibody will only recognize folded GFP and therefore will not work in Western blotting.

Our recombinant anti-GFP antibody binds strongly to most fluorescent proteins derived from Aequorea victoria. Explore our FP Specificity Chart for compatibility information.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Rabbit IgG Fc-fusion 100 µg N0383 AB_3075913
Related Products:

FluoTag®-Q anti-GFP (Cat. No. N0301)

FluoTag®-X4 anti-GFP (Cat. No. N0304)

sdAb anti-GFP (Cat. No. N0305)

GFP Selector (Cat. No. N0310; N0315)

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Clone: 1H1
Host: Alpaca
Produced in: HEK
Application: IF, IHC
Dilution: 1:500 (IF, IHC)
Capacity: N/A
Antigen: -
Targets: GFP
Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Formulation:

Lyophilized from PBS pH 7.4. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.
Limited Use Label License: Internal Research Use Only
The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.