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N4183_Recombinant anti-CNPase antibody_Rabbit IgG Fc-fusion_NanoTag

Indirect immunostaining of PFA-fixed sagittal rat hippocampus section using Recombinant anti-CNPase antibody Rabbit IgG Fc-fusion (Cat. No. N4183; 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Rabbit IgG Atto643 (Cat. No. N2402-At643, 1:500; green). Cell nuclei are counterstained with DAPI (blue). Scale bar: 50 µm.

 N4182_Recombinant anti-CNPase antibody_Mouse IgG1 Fc-fusion_NanoTag

Indirect immunostaining of PFA-fixed sagittal rat hippocampus section using Recombinant anti-CNPase antibody Mouse IgG1 Fc-fusion (Cat. No. N4182; 1:1000), detected with Smart Secondary® – FluoTag®-X2 anti-Mouse IgG1 AZDye568 (Cat. No. N2002-AF568, 1:500; green). Cell nuclei are counterstained with DAPI (blue). Scale bar: 50 µm.
N4183_Recombinant anti-CNPase antibody_Rabbit IgG Fc-fusion_NanoTag
N4182_Recombinant anti-CNPase antibody_Mouse IgG1 Fc-fusion_NanoTag

Recombinant anti-CNPase antibody (rHcAb)

Cat No: N/A Category:

420,00 €

The recombinant heavy-chain anti-CNPase antibody is a genetic fusion of our established camelid anti-CNPase single-domain antibody (sdAb) to either a Mouse IgG1 or a Rabbit IgG Fc domain. It specifically recognizes mouse and rat CNPase.

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The recombinant heavy-chain anti-CNPase antibody is a genetic fusion of our established camelid anti-CNPase single-domain antibody (sdAb) to either a Mouse IgG1 or a Rabbit IgG Fc domain. The resulting bivalent binders combine the high target specificity of sdAbs with maximal flexibility and coherence needed for Fc-domain-based applications. The antibody specifically recognizes mouse and rat CNPase. It can be detected using conventional secondary reagents or with our secondary nanobodies (Smart Secondaries®), which offer advantages in applications where secondary antibody clustering is a concern or where a smaller probe size is required. More information on recombinant heavy-chain antibodies (rHcAb) can be found here.

CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase), also known as CNP1, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system. It may also be involved in the RNA metabolism of myelinating cells of the central nervous system (CNS), the oligodendrocytes. CNPase is frequently used as a marker for oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS).

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Rabbit IgG Fc-fusion 100 µg N4183 AB_3741787
Mouse IgG1 Fc-fusion 100 µg N4182 AB_3741786
Related Products:

FluoTag®-X2 anti-CNPase (Cat. No. N4102)

sdAb anti-CNPase (Cat. No. N4105)

View related products
Clone: 1D5
Host: Alpaca
Produced in: HEK
Application: IF, IHC
Dilution: 1:500 (IF), 1:1000 or 1 µg/mL (IHC)
Capacity: N/A
Antigen: CNPase
Targets: CNPase
Specificity: Recognizes mouse and rat CNPase. Other species not tested. Based on high sequence similarity, it may also recognize human CNPase.
Formulation:

Lyophilized from PBS pH 7.4. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.

Limited Use Label License: Internal Research Use Only
The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.