Contact Shop

COS-7 cell transfected with Vimentin-ALFA stained with Recombinant heavy-chain anti-ALFA antibody (Cat. No. N1583, dilution 1:500) and FluoTag-X4 anti-rabbit IgG Atto 488 (Cat. No N2404-At488-S). Nuclei were visualized using DAPI.

Recombinant anti-ALFA antibody (rHcAb)

Cat No: N/A Category:

420,00 €

The recombinant heavy-chain anti-ALFA antibody is a genetic fusion of our established camelid anti-ALFA single-domain antibody (sdAb) to various IgG Fc domains of the most commonly employed host species.

Read more

The recombinant heavy-chain anti-ALFA antibody is a genetic fusion of our established camelid anti-ALFA single-domain antibody (sdAb) to various IgG Fc domains of the most commonly employed host species. This antibody preserves the specificity and high-affinity binding of the anti-ALFA single-domain antibody and adds the features of a conventional IgG type antibody. It can be used in Western blotting and many other applications based on immunodetection (IF, IHC, IP). More information on recombinant heavy-chain antibodies (rHcAb) can be found here.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Mouse IgG1 Fc-fusion 100 µg N1582 AB_3065196
Rabbit IgG Fc-fusion 100 µg N1583 AB_3075998
Mouse IgG2 Fc-fusion 100 µg N1585 AB_3076000
Guinea Pig IgG Fc-fusion 100 µg N1584 AB_3075999
Human IgG1 Fc-fusion 100 µg N1586 AB_3076001
Related Products:

FluoTag®-X2 anti-ALFA (Cat. No. N1502)

sdAb anti-ALFA (Cat. No. N1505)

View related products
Clone: 1G5
Host: Alpaca
Produced in: HEK
Application: IF, IHC, WB
Dilution: 1:500 (IF, IHC), 1:1000 WB
Capacity: N/A
Antigen: -
Targets: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE).
kDa: -
Ext Coef: -
Formulation:

Lyophilized from PBS pH 7.4. Reconstitute with 100 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. Basak S, Inamdar K, Pollack YG, et al. Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy. Nano Lett. 2026;26(35):11808-11814. doi:10.1021/acs.nanolett.6c02799 (N1582; ICC/IF; dSTORM; COS-7 cells)
  3. Boswell CW, Hoppe C, Sherrard A, et al. Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo. Nat Commun. 2025;16(1):5503. Published 2025 Jul 1. doi:10.1038/s41467-025-61003-w (#N1583; IF/IHC, ChIP-seq, WB; zebrafish)
  4. Hoffmann MD, Sorensen RJ, Extross A, He Y, Schmidt D. Protein Carrier Adeno-Associated Virus. ACS Nano. 2025;19(12):12308-12322. doi:10.1021/acsnano.5c01498 (N1582; WB; AAV)
  5. Bonanno SL, Sanfilippo P, Eamani A, et al. Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors in Drosophila. J Neurosci. 2024;44(33):e2377232024. Published 2024 Aug 14. doi:10.1523/JNEUROSCI.2377-23.2024 (IHC; fruit fly)
  6. Potlapalli BP, Fuchs J, Rutten T, Meister A, Houben A. The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit. J Exp Bot. 2024;75(20):6244-6257. doi:10.1093/jxb/erae341 (CRISPR-FISH; DNA repeats/centromere labeling, A. thaliana; telomere labeling, N. benthamiana)
  7. Kim YJ, Tohyama S, Nagashima T, et al. A light-controlled phospholipase C for imaging of lipid dynamics and controlling neural plasticity. Cell Chem Biol. 2024;31(7):1336-1348.e7. doi:10.1016/j.chembiol.2024.03.001 (#N1582; WB; HEK293T)
  8. Andreu-Carbó M, Egoldt C, Velluz MC, Aumeier C. Microtubule damage shapes the acetylation gradient. Nat Commun. 2024;15(1):2029. Published 2024 Mar 6. doi:10.1038/s41467-024-46379-5 (N1586; IF/ICC; HeLa)
  9. Frodyma DE, Troia TC, Rao C, et al. PGC-1β and ERRα Promote Glutamine Metabolism and Colorectal Cancer Survival via Transcriptional Upregulation of PCK2. Cancers (Basel). 2022;14(19):4879. Published 2022 Oct 5. doi:10.3390/cancers14194879 (WB; human HCT116, HT-29)
  10. Nozawa K, Sogabe T, Hayashi A, et al. In vivo nanoscopic landscape of neurexin ligands underlying anterograde synapse specification. Neuron. 2022;110(19):3168-3185.e8. doi:10.1016/j.neuron.2022.07.027 (IHC; mouse brain)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.
Product LULL: Limited Use Label License (LULL) – Internal Research Use Only applies.