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Human B-cell Activator__hBCA_N1170_NanoTag Biotechnologies

Ligand-induced stimulation of the B-cell antigen receptor (BCR) in human B cells. Ramos cells pre-loaded with the Ca2+ indicator dye Indo1-AM were treated with a tetrameric Human B-cell Activator (hBCA, Cat. No. N1170, blue curve), or the respective monomeric reagent (sdAb anti-Human IgM, Cat. No. N1101, grey curve). An anti-Human Fc5µ-specific F(ab′)2 fragment from goat served as a reference (red curve). All reagents were used at a concentration of 5 µg/mL. The stimulation time point is indicated by an arrow head. The BCR stimulation capacity of the different reagents was compared by a flow cytometric analysis of cytosolic Ca2+ mobilization kinetics. The tetrameric Human B-cell Activator (hBCA) leads to stronger and quicker Ca2+ mobilization than the reference reagent. Ca2+ mobilization is not observed when using the monomeric sdAb anti-Human IgM. Image courtesy of Niklas Engels, UMG Göttingen, Germany.
Human B-cell Activator__hBCA_N1170_NanoTag Biotechnologies

Human B-cell Activator (hBCA)

Cat No: N1170 Category:

290,00 €

Human B-cell Activator (hBCA) is a tetramerized single-domain antibody (sdAb) that crosslinks surface-bound human IgM to drive rapid BCR clustering and efficient B-cell activation.

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Human B-cell Activator (hBCA) is a tetramerized single-domain antibody (sdAb) specifically designed to crosslink surface-bound human IgM (BCR μ-chain) and promote efficient nanoscale receptor clustering. This clustering drives rapid proximal signaling cascades, including phosphorylation events, calcium flux, and MAPK/NF-κB activation.

By leveraging a tetrameric sdAb format, the reagent provides robust BCR clustering without the need for secondary antibodies and consistently yields a faster and stronger activation response compared with conventional IgM crosslinking antibodies. The Fc-free design avoids Fc receptor–mediated artifacts. Recombinant production guarantees identical performance with minimal batch-to-batch variations.

Human B-cell Activator_hBCA_Figure_NanoTag Biotechnologies

The Human B-cell Activator is compatible with primary human B cells and IgM⁺ B-cell lines, and it shows no cross-reactivity with mouse or rat IgM. It supports a broad range of research applications, including signaling pathway analysis, activation marker profiling, BCR clustering imaging, proliferation assays, cytokine readouts, and functional co-culture models. A detailed manual is available under the Protocol button below.

MSDS
Protocols
Reconstitution and Storage
Manual Human B-cell Activator
Variations:
Conjugation Amount Cat No. RRID
Tetramerization domain 250 μg N1170 AB_3717737
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Clone: 2F2
Host: Alpaca
Produced in: E.coli
Application:

Cellular assays
Dilution: To be determined (0.5-10 μg/mL)
Capacity: N/A
Antigen: Human IgM
Targets: Human IgM
Specificity: Specific for Human IgM, no known cross-reactivity to other Human immunoglobulins or IgM from other species.
Formulation:

Lyophilized from PBS. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 20.1 kDa (monomer); 80.4 kDa (tetramer)
Ext Coef: 23045 (monomer); 91280 (tetramer)
Shipping: Ambient temperature
Storage:

Vials containing lyophilized protein can be stored at 4°C for up to 12 months. After reconstitution, working aliquots can be stored at -20°C for up to 4 weeks. Long term storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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