Halo Selector Resins_2ml_N5010_N5015_HaloTag Immunoprecipitation (IP)_NanoTag Biotechnologies

Halo Selector Resin_N5015_Nanotag Biotechnologies

One-step affinity purification using the magnetic agarose Halo Selector Resin (Cat. No. N5015). A 2.5 µM purified mNeonGreen-Halo fusion protein (left lane) was blended into E. coli lysate and incubated with the magnetic Halo Selector Resin. Samples were taken before incubation (Input) and after incubation (Non-bound). Bound proteins were eluted with SDS sample buffer. Indicated fractions were analyzed by SDS–PAGE followed by Coomassie Blue staining. The eluate fraction corresponds to the material eluted from 1 μL of resin. B Control experiment performed without target protein.

Halo Selector

Name: Halo Selector
SKU: N5010
Availability: InStock

Cat No: N5010 Categories: Affinity Resins, Selector Resins

Price range: 290,00 € through 465,00 €

Halo Selectors are specialized affinity resins designed for the efficient purification and enrichment of Halo-tagged proteins. They feature a high-affinity single-domain antibody (sdAb) that specifically recognizes the HaloTag^®. The resins are available as agarose or magnetic agarose beads.

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The HaloTag^®⁽¹⁾technology is a widely used and highly versatile platform in molecular and cellular biology, enabling detailed analyses of protein function, dynamics, and molecular interactions. It is based on a genetically engineered haloalkane dehalogenase derived from bacteria, which has been modified to form a specific and stable covalent bond with synthetic Halo ligands.

Halo Selectors are specialized affinity resins designed for the efficient purification and enrichment of Halo-tagged proteins of interest (POIs). They feature a high-affinity single-domain antibody (sdAb) that specifically recognizes the HaloTag^®. Binding of Halo fusion proteins to Halo Selectors typically does not interfere with the interaction of the HaloTag^® with Halo ligands, thereby preserving the functional integrity of the fusion protein during purification or assay workflows.

Halo Selector Resin_NanoTag Biotechnologies

Halo Selector Resins are available in both agarose or magnetic agarose bead formats. The site-directed and chemically stable immobilization of target-specific sdAbs ensures high capacity and optimal accessibility of available binding sites. At the same time, minimal leakage of sdAbs is observed, even under harsh denaturing and/or reducing conditions. Due to their combination of high capacity, minimal leakage and extraordinarily low non-specific protein adsorption, Halo Selector Resins enable clean and highly specific immunoprecipitations.

Halo Selectors are broadly compatible not only with physiological buffers and most common lysis/washing buffers, but also with high stringency buffers, detergents, and reducing agents. The resins can therefore be used in customized applications employing a wide variety of buffer compositions and pH values. As guidance, please refere to some suggestions provided in the Halo Selector Manual.

⁽¹⁾ HaloTag^® is a registered trademark of Promega Corporation.

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